Document Detail


Free and conjugated catecholamines in dog pulmonary artery: presence and pharmacological action.
MedLine Citation:
PMID:  2886059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Free and conjugated forms of norepinephrine (NE) and dopamine (DA) were detected in dog pulmonary artery, and small amounts were released during 2-Hz electrical stimulation. Hydrolysis of the conjugates by acid boiling and by enzymes indicated that they were primarily sulfates, but traces of glucuronide may also have been present. After isolation by column chromatography, free and conjugated amines were quantitated using liquid chromatography with electrochemical detection. Pulmonary artery tissue contained (in ng) 1,240 +/- 100 free NE, 15 +/- 5 conjugated NE, 47 +/- 6 free DA, and 2 +/- 0.3 conjugated DA/g tissue (means +/- SE). When strips of pulmonary artery were immersed in [3H]NE small amounts of [3H]NE sulfate were synthesized. The release of conjugated NE was not calcium dependent but release was attenuated somewhat by tetrodotoxin. Removal of endothelium did not change tissue content of conjugated or of free NE nor the amounts measured in superfusate. Small amounts of free DA were also detected in tissues and in superfusate during stimulation, but only when calcium was present in the superfusion fluid. Norepinephrine sulfate was not a potent agonist at either pre- or postsynaptic alpha-receptors; the agonist activity seen with high concentrations of norepinephrine sulfate seems likely to have been due at least in part to trace contamination by free NE.
Authors:
D K Rorie; G M Tyce
Related Documents :
2847509 - Effects of bradykinin and leukotrienes b4 and d4 on the bovine bronchial artery in vitr...
11243419 - Increased sensitivity of vascular smooth muscle to nitric oxide in dilated cardiomyopat...
11212019 - In vitro pharmacologic effect of two endothelin-1 antagonists on equine colonic arterie...
11498459 - Ace genotype and endothelium-dependent vasodilation of conduit arteries and forearm mic...
23087399 - Prospective characterization of catheter-tissue contact force at different anatomical s...
1934359 - Chronic treatment with polyethylene-glycolated superoxide dismutase partially restores ...
22893319 - Isolated aneurysm of the proximal right subclavian artery treated with consideration to...
22363909 - Coronary-bronchial artery fistula manifested by hemoptysis and myocardial ischemia in a...
11868099 - Coronary artery aneurysm and type-a aortic dissection demonstrated by retrospectively e...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  253     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-08-18     Completed Date:  1987-08-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H66-74     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adrenergic alpha-Agonists / pharmacology
Amines / metabolism
Animals
Calcium / pharmacology
Dogs
Dopamine / metabolism*
Electric Stimulation
Endothelium / metabolism,  physiology
Female
Male
Norepinephrine / analogs & derivatives,  metabolism*,  pharmacology
Pulmonary Artery / metabolism*
Synapses / physiology
Tetrodotoxin / pharmacology
Grant Support
ID/Acronym/Agency:
HL-23217/HL/NHLBI NIH HHS; NS-17858/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Amines; 4368-28-9/Tetrodotoxin; 51-41-2/Norepinephrine; 7440-70-2/Calcium; 81138-41-2/noradrenaline sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Increased vascular resistance during complement-activated plasma infusion in swine.
Next Document:  Inhibition of renal sympathetic nervous activity by area postrema stimulation in rabbits.