Document Detail

Free and conjugated catecholamines in dog pulmonary artery: presence and pharmacological action.
MedLine Citation:
PMID:  2886059     Owner:  NLM     Status:  MEDLINE    
Free and conjugated forms of norepinephrine (NE) and dopamine (DA) were detected in dog pulmonary artery, and small amounts were released during 2-Hz electrical stimulation. Hydrolysis of the conjugates by acid boiling and by enzymes indicated that they were primarily sulfates, but traces of glucuronide may also have been present. After isolation by column chromatography, free and conjugated amines were quantitated using liquid chromatography with electrochemical detection. Pulmonary artery tissue contained (in ng) 1,240 +/- 100 free NE, 15 +/- 5 conjugated NE, 47 +/- 6 free DA, and 2 +/- 0.3 conjugated DA/g tissue (means +/- SE). When strips of pulmonary artery were immersed in [3H]NE small amounts of [3H]NE sulfate were synthesized. The release of conjugated NE was not calcium dependent but release was attenuated somewhat by tetrodotoxin. Removal of endothelium did not change tissue content of conjugated or of free NE nor the amounts measured in superfusate. Small amounts of free DA were also detected in tissues and in superfusate during stimulation, but only when calcium was present in the superfusion fluid. Norepinephrine sulfate was not a potent agonist at either pre- or postsynaptic alpha-receptors; the agonist activity seen with high concentrations of norepinephrine sulfate seems likely to have been due at least in part to trace contamination by free NE.
D K Rorie; G M Tyce
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  253     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-08-18     Completed Date:  1987-08-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H66-74     Citation Subset:  IM    
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MeSH Terms
Adrenergic alpha-Agonists / pharmacology
Amines / metabolism
Calcium / pharmacology
Dopamine / metabolism*
Electric Stimulation
Endothelium / metabolism,  physiology
Norepinephrine / analogs & derivatives,  metabolism*,  pharmacology
Pulmonary Artery / metabolism*
Synapses / physiology
Tetrodotoxin / pharmacology
Grant Support
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Amines; 4368-28-9/Tetrodotoxin; 51-41-2/Norepinephrine; 7440-70-2/Calcium; 81138-41-2/noradrenaline sulfate

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