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Free amino acid profiling in grain Amaranth using LC-MS/MS.
MedLine Citation:
PMID:  23442725     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Reliable and reproducible techniques for identification and quantification of amino acids usually require derivatization. However, techniques such as LC-MS/MS may perhaps sideline the derivatization with significant accuracy. Understanding the interaction between free amino acids and several physiological processes apparently requires that the pool be studied in the seed itself. Data on free amino acid content of grain amaranth is very inadequate. The present analysis revealed a higher concentration of essential amino acids especially Threonine and Tryptophan than non essential amino acids. The study constitutes first report of use of LC-MS/MS method for analysing the free amino acid composition of amaranth grain.
Authors:
Mansingraj S Nimbalkar; Sandeep R Pai; Nilesh V Pawar; Dashrath Oulkar; Ghansham B Dixit
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Publication Detail:
Type:  Journal Article     Date:  2012-04-19
Journal Detail:
Title:  Food chemistry     Volume:  134     ISSN:  0308-8146     ISO Abbreviation:  Food Chem     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2013-02-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702639     Medline TA:  Food Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  2565-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Laboratory of Cytogenetics and Plant Breeding, Department of Botany, Shivaji University, Kolhapur 416 004, Maharashtra, India; Sahyadri Group for Enriching Nature, Environment and Science (S-GENES), 2113 C, 26 A, Plot No. 8 and 9, Near Rajani Residency, Behind Rajesh Motors, Kawla Naka, Kolhapur 416 003, Maharashtra, India.
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