Document Detail


Free amino acid content of lymphocytes nd granulocytes compared.
MedLine Citation:
PMID:  7094296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For extracting free amino acids from human leukocytes, we find that disruption of the cells by ultrasonication is more reliable than freezing and thawing. The amount of free amino acids extracted by the latter method depends on the temperature and duration of thawing. We extracted free amino acids from leukocytes of healthy men by ultrasonication and compared their concentrations in lymphocytes and granulocytes. The amino acid content of granulocytes significantly (p less than 0.001) exceeded that of lymphocytes. Of the amino acids extracted from granulocytes, 76% was taurine; for lymphocytes taurine, aspartic acid, and glutamic acid were predominant, respectively composing 44%, 17%, and 26% of the total. Taurine is proposed as an index of cell disruption.
Authors:
K Fukuda; Y Hirai; H Yoshida; T Nakajima; T Usui
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  28     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1982 Aug 
Date Detail:
Created Date:  1982-09-17     Completed Date:  1982-09-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1758-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Amino Acids / analysis*
Cold Temperature
Granulocytes / analysis*
Humans
Lymphocytes / analysis*
Male
Reference Values
Sonication
Taurine / analysis*
Chemical
Reg. No./Substance:
0/Amino Acids; 107-35-7/Taurine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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