Document Detail

Fragmentation of protonated ions of peptides containing cysteine, cysteine sulfinic acid, and cysteine sulfonic acid.
MedLine Citation:
PMID:  15121199     Owner:  NLM     Status:  MEDLINE    
The oxidation of the sulfhydryl group in cysteine to sulfenic acid, sulfinic acid, and sulfonic acid in proteins is important in a number of enzymatic processes. In this study we examined the fragmentation of four peptides containing cysteine, cysteine sulfinic acid (Cys-SO(2)H), and cysteine sulfonic acid (Cys-SO(3)H) in an ion-trap mass spectrometer. Our results show that the presence of a Cys-SO(2)H in a peptide leads to preferential cleavage of the amide bond at the C-terminal side of the oxidized cysteine residue. The results are important for the determination of the site of the cysteine oxidation and might be useful for the sequencing of cysteine-containing peptides.
Yinsheng Wang; Shetty Vivekananda; Lijie Men; Qibin Zhang
Related Documents :
5500319 - The effect of hypermethylation on the functional properties of transfer ribonucleic aci...
649259 - Solid-phase peptide synthesis using mild base cleavage of n alpha-fluorenylmethyloxycar...
10565789 - Organometallic 99mtc-aquaion labels peptide to an unprecedented high specific activity.
22270809 - Equipotent inhibition of fatty acid amide hydrolase and monoacylglycerol lipase - dual ...
8765519 - Mechanistic studies on the inactivation of papain by epoxysuccinyl inhibitors.
18020949 - Overexpression and mass spectrometry analysis of mature human acid ceramidase.
20071749 - Zip nucleic acids are potent hydrolysis probes for quantitative pcr.
16348909 - Metabolic pathways in methanococcus jannaschii and other methanogenic bacteria.
17906979 - Thiazolinium and imidazolium chiral ionic liquids derived from natural amino acid deriv...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  15     ISSN:  1044-0305     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-03     Completed Date:  2004-06-18     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  697-702     Citation Subset:  IM    
Department of Chemistry, University of California at Riveirside, Riverside, California 92521-0403, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Cysteine / analogs & derivatives*,  chemistry*
Ions / chemistry
Molecular Sequence Data
Molecular Structure
Neurotransmitter Agents
Peptides / chemistry*
Sulfonic Acids / chemistry*
Reg. No./Substance:
0/Ions; 0/Neurotransmitter Agents; 0/Peptides; 0/Protons; 0/Sulfonic Acids; 2381-08-0/cysteine sulfinic acid; 52-90-4/Cysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cleavage reactions of the complex ions derived from self-complementary deoxydinucleotides and alkali...
Next Document:  De novo sequencing, peptide composition analysis, and composition-based sequencing: a new strategy e...