Document Detail

Fragmentation of protonated ions of peptides containing cysteine, cysteine sulfinic acid, and cysteine sulfonic acid.
MedLine Citation:
PMID:  15121199     Owner:  NLM     Status:  MEDLINE    
The oxidation of the sulfhydryl group in cysteine to sulfenic acid, sulfinic acid, and sulfonic acid in proteins is important in a number of enzymatic processes. In this study we examined the fragmentation of four peptides containing cysteine, cysteine sulfinic acid (Cys-SO(2)H), and cysteine sulfonic acid (Cys-SO(3)H) in an ion-trap mass spectrometer. Our results show that the presence of a Cys-SO(2)H in a peptide leads to preferential cleavage of the amide bond at the C-terminal side of the oxidized cysteine residue. The results are important for the determination of the site of the cysteine oxidation and might be useful for the sequencing of cysteine-containing peptides.
Yinsheng Wang; Shetty Vivekananda; Lijie Men; Qibin Zhang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  15     ISSN:  1044-0305     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-03     Completed Date:  2004-06-18     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  697-702     Citation Subset:  IM    
Department of Chemistry, University of California at Riveirside, Riverside, California 92521-0403, USA.
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MeSH Terms
Amino Acid Sequence
Cysteine / analogs & derivatives*,  chemistry*
Ions / chemistry
Molecular Sequence Data
Molecular Structure
Neurotransmitter Agents
Peptides / chemistry*
Sulfonic Acids / chemistry*
Reg. No./Substance:
0/Ions; 0/Neurotransmitter Agents; 0/Peptides; 0/Protons; 0/Sulfonic Acids; 2381-08-0/cysteine sulfinic acid; 52-90-4/Cysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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