Document Detail


Fragile X repeats are potent inducers of complex, multiple site rearrangements in flanking sequences in Escherichia coli.
MedLine Citation:
PMID:  17851139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
(CGG.CCG)n repeats induce the formation of complex, multiple site rearrangements and/or gross deletions in flanking DNA sequences in Escherichia coli plasmids. DNA sequence analyses of mutant clones revealed the influence of (a) the length (24, 44 or 73 repeats), (b) the orientation of the CGG.CCG region relative to the unidirectional origin, and (c) its transcription status. Complex rearrangements had occurred in the mutant clones since some products contained deletions, inversions and insertions and some products had only gross deletions. Furthermore, the CGG.CCG repeats repeatedly induced, up to 22 times, the formation of identical (to the bp) mutagenic products indicating the powerful nature of the complex processes involved. Also, the mutations were bidirectional from the CGG.CCG tract. The healed junctions had CG-rich microhomologies of 1-6bp, CG-rich regions and putative cruciforms and slipped structures. Hence, the fragile X syndrome mutagenic spectrum has been found, at least in part, in our model system.
Authors:
Beata Kosmider; Robert D Wells
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-09-11
Journal Detail:
Title:  DNA repair     Volume:  6     ISSN:  1568-7864     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-02-05     Revised Date:  2008-07-30    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1850-63     Citation Subset:  IM    
Affiliation:
Center for Genome Research, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Texas Medical Center, 2121 W. Holcombe Blvd., Houston, TX 77030-3303, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA, Bacterial
Escherichia coli / genetics*
Fragile X Syndrome / genetics*
Gene Rearrangement*
Lac Operon
Plasmids
Repetitive Sequences, Nucleic Acid*
Transcription, Genetic
Transformation, Genetic
Grant Support
ID/Acronym/Agency:
ES11347/ES/NIEHS NIH HHS; NS37554/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections
Comment In:
DNA Repair (Amst). 2008 May 3;7(5):684-5   [PMID:  18337192 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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