Document Detail


FRACTIONATION OF THYMIDINE PHOSPHOKINASE, THYMIDINE 5'-MONOPHOSPHATE PHOSPHOKINASE AND THYMIDINE 5'-DIPHOSPHATE PHOSPHOKINASE IN EXTRACTS OF LANDSCHUTZ ASCITES-TUMOUR CELLS.
MedLine Citation:
PMID:  14340042     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina C(gamma) gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5'-monophosphate phosphokinase and thymidine 5'-diphosphate phosphokinase. 2. These results clearly demonstrate the existence in Landschutz ascites tumour of three phosphokinases each of which catalyses one step in the reaction sequence: thymidineright harpoon over left harpoonthymidine 5'-monophosphateright harpoon over left harpoonthymidine 5'-diphosphateright harpoon over left harpoonthymidine 5'-triphosphate. Though these results do not preclude the participation of other enzymes in the formation of thymidine 5'-triphosphate from thymidine by Landschutz ascites-tumour cells, they provide strong support for the view that thymidine 5'-diphosphate is an intermediate in the formation of thymidine 5'-triphosphate from thymidine 5'-monophosphate by this system.
Authors:
H J GRAV; R M SMELLIE
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  94     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1965 Mar 
Date Detail:
Created Date:  1965-12-01     Completed Date:  1996-12-01     Revised Date:  2011-09-28    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  518-24     Citation Subset:  OM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biochemical Phenomena
Biochemistry*
Chemistry Techniques, Analytical*
Chromatography*
Chromatography, Gel*
Dextrans*
Nucleotides*
Research*
Spectrophotometry*
Thymidine Kinase*
Tissue Culture Techniques*
Chemical
Reg. No./Substance:
0/Nucleotides; 9004-54-0/Dextrans; EC 2.7.1.21/Thymidine Kinase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  THE ISOLATION OF AMINO ACIDS FROM MIXTURES AS THEIR N-2,4-DINITROPHENYL DERIVATIVES, METHIONINE, LEU...
Next Document:  THE ISOLATION OF OLIGOSACCHARIDES FROM THE CELL-WALL POLYSACCHARIDE OF LACTOBACILLUS CASEI, SEROLOGI...