| Fractionation and kinetic properties of rat liver and kidney methionine adenosyltransferase isozymes. | |
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MedLine Citation:
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PMID: 6849873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three isozymes of methionine adenosyltransferase (EC 2.5.1.6; MAT-I, -II, and -III) exist in normal rat liver and are conveniently purified (MAT-III to homogeneity) by a three-step column chromatography procedure. MAT-I shows Michaelis-Menten kinetics with a Km (L-methionine) of 41 microM and a molecular weight of 208 000 and is slightly inhibited by S-adenosyl-L-methionine (Adomet). MAT-II, which is also the only isozyme found in normal rat kidney, shows negative cooperativity with a Hill coefficient of 0.7. It has a L-methionine concentration required for half-maximal velocity [S0.5(Met)] of 8 microM and a molecular weight of 120 000 and is strongly inhibited by Adomet. MAT-I and -II comprise 15% and 5%, respectively, of total MAT activity in rat liver. The predominant isozyme in rat liver, MAT-III, demonstrates positive cooperativity with a Hill coefficient of 1.8. It has a molecular weight of 97 000 and apparently consists of two subunits of identical molecular weight (47 000). This liver-specific isozyme is strongly activated by both dimethyl sulfoxide and Adomet and has a S0.5(Met) of 215 microM. |
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Authors:
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D M Sullivan; J L Hoffman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 22 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-07-08 Completed Date: 1983-07-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1636-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Ion Exchange Female Isoenzymes / isolation & purification*, metabolism Kidney / enzymology* Kinetics Liver / enzymology* Methionine Adenosyltransferase / isolation & purification*, metabolism Molecular Weight Rats Rats, Inbred Strains Transferases / isolation & purification* |
| Grant Support | |
ID/Acronym/Agency:
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CA 22166/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 2.-/Transferases; EC 2.5.1.6/Methionine Adenosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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