Document Detail


Fractionation of carbohydrates in Arabidopsis root cell walls shows that three radial swelling loci are specifically involved in cellulose production.
MedLine Citation:
PMID:  10987560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three non-allelic radial swelling mutants (rsw1, rsw2 and rsw3) of Arabidopsis thaliana L. Heynh. were shown to be specifically impaired in cellulose production. Fractionation methods that identify, characterise and quantify some of the major cell wall polysaccharides in small quantities of seedlings demonstrated that changes in the production of cellulose are much more pronounced than changes in the production of non-cellulosic polysaccharides. A crude cell wall pellet was sequentially extracted with chloroform methanol (to recover lipids), dimethyl sulphoxide (starch), ammonium oxalate (pectins) and alkali (hemicelluloses). Crystalline cellulose remained insoluble through subsequent treatments with an acetic/nitric acid mixture and with trifluoroacetic acid. Cetyltrimethylammonium bromide precipitation resolved neutral and acidic polymers in the fractions, and precipitation behaviour, monosaccharide composition and glycosidic linkage patterns identified the major polysaccharides. The deduced composition of the walls of wild-type seedlings and the structure and solubility properties of the major polymers were broadly typical of other dicots. The three temperature-sensitive, radial swelling mutants produced less cellulose in their roots than the wild type when grown at their restrictive temperature (31 degrees C). There were no significant differences at 21 degrees C where no radial swelling occurs. The limited changes seen in the monosaccharide compositions, glycosidic linkage patterns and quantities of non-cellulosic polysaccharides support the view that the RSW1, RSW2 and RSW3 genes are specifically involved in cellulose synthesis. Reduced deposition of cellulose was accompanied by increased accumulation of starch.
Authors:
L Peng; C H Hocart; J W Redmond; R E Williamson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Planta     Volume:  211     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2001-01-11     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  406-14     Citation Subset:  IM    
Affiliation:
Plant Cell Biology Group, Research School of Biological Sciences, Institute of Advanced Studies, Australian National University, Canberra, ACT.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology,  genetics,  metabolism*
Carbohydrate Metabolism*
Carbohydrates / isolation & purification
Cellulose / biosynthesis*
Colorimetry
Gas Chromatography-Mass Spectrometry
Plant Roots / cytology,  metabolism*
Polysaccharides / biosynthesis*
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Polysaccharides; 9004-34-6/Cellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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