Document Detail


Fractionated dosing of cyclophosphamide for establishing long-lasting skin allograft survival, stable mixed chimerism, and intrathymic clonal deletion in mice primed with allogeneic spleen cells.
MedLine Citation:
PMID:  9197364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Injection of allo-spleen cells (SC) followed by a single dose of cyclophosphamide (CP) can induce tolerance of tumor and/or skin allografts in mice. To minimize the damage caused by CP, fractionation of CP that can establish long-lasting skin graft survival, stable mixed chimerism, and intrathymic clonal deletion in the host was investigated in the present study. METHODS: Allo-SC (10(8)) were given intravenously on day 0. CP at 200 mg/kg was given intraperitoneally on day 2 in a single dose (CP 200x1 group). CP at 100, 66, 50, 40, and 33 mg/kg was given daily from day 1 through days 2, 3, 4, 5 and 6, respectively, in the fractionated doses (CP 100x2, 66x3, 50x4, 40x5, and 33x6 groups; total dose=200 mg/kg). Allografting was performed on day 14. RESULTS: In a fully allogeneic combination of C57BL/6 (H2b)-->AKR (H2k, Mls-1a), an EL-4 tumor (H2b) was specifically accepted to kill the AKR mice in all of the SC+CP 200x1, 100x2, 66x3, 50x4, 40x5, and 33x6 groups (n=6), but C57BL/6 skin graft survival was not prolonged in any of the tumor-tolerant groups. In an H2-identical combination of AKR-->C3H (H2k, Mls-1b), AKR skin graft survival was prolonged remarkably (80-90 days) in the SC+CP 200x1, 100x2, and 66x3 groups (n=5-11), but was prolonged moderately (20-60 days) in the SC+CP 50x4 and 40x5 groups. In both of the SC+CP 200x1 and 66x3 groups in the AKR-->C3H combination, mixed chimerism was maintained for as long as 100 days after tolerance induction in both the spleen and thymus, associated with intrathymic clonal deletion of Vbeta6+ T cells. The decreases in leukocyte count, hemoglobin level, spleen weight, SC count, and body weight were significantly smaller in the SC+CP 66x3 group than in the SC+CP 200x1 group. CONCLUSIONS: Fractionated CP is effective in ameliorating the compromised state induced by a single dose of CP. To induce a long-lasting skin allograft survival associated with stable mixed chimerism and intrathymic clonal deletion in an H2-identical combination, 200 mg/kg of CP can be divided into three or fewer fractions.
Authors:
Q W Zhang; H Mayumi; M Umesue; Y Tomita; K Nomoto; H Yasui
Related Documents :
715814 - Effect of gamma ray low doses on the system of the nonspecific defense of the organism.
6869534 - Developmental pattern of water and electrolyte transport in rat superficial nephrons.
25001274 - Dose-dependent arterial destiffening and inward remodeling after olmesartan in hyperten...
16663034 - Hemicellulosic polymers of cell walls of zea coleoptiles.
3988394 - Pharmacokinetics of pheniramine (avil) and metabolites in healthy subjects after oral a...
16726154 - Immunosuppressive activity of uterine luminal protein from steroid-treated ovariectomiz...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation     Volume:  63     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-07-09     Completed Date:  1997-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1667-73     Citation Subset:  IM    
Affiliation:
Research Institute of Angiocardiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Clonal Deletion / physiology*
Cyclophosphamide / administration & dosage*
Drug Administration Schedule
Female
Graft Survival / drug effects
Leukopenia / prevention & control
Mice
Mice, Inbred AKR
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred CBA
Skin Transplantation / immunology*
Spleen / cytology*
Thymus Gland / cytology*
Transplantation Chimera / physiology*
Transplantation Conditioning*
Chemical
Reg. No./Substance:
50-18-0/Cyclophosphamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Prevention of cyclosporine-induced nephrotoxicity with dietary glycine.
Next Document:  Removal of xenoreactive human anti-pig antibodies by absorption on recombinant mucin-containing glyc...