Document Detail


Fphog1, a HOG-type MAP kinase gene, is involved in multistress response in Fusarium proliferatum.
MedLine Citation:
PMID:  18506895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Delta Fphog1 mutants of Fusarium proliferatum obtained by targeted gene disruption of Fphog1, an orthologue of the Saccharomyces cerevisiae hog1 MAPK gene showed increased sensitivity towards different abiotic stressors including UV-irradiation, heat, salt, osmotic and hydrogen peroxide treatments. Incubation of the Delta Fphog1 mutants under hyperosmotic conditions was accompanied with prolonged growth arrest, inhibition of conidial germination, morphological abnormalities and time-dependent increase of the cell death rate. The wild type Fphog1 gene, under the control of its own promoter, was able to rescue the multistress sensitivity of the mutant strain. Real time qPCR data demonstrated that under salt and sorbitol stress conditions the Fphog1 gene is not subject of transcriptional regulation. Levels of reactive oxygen species (ROS), mitochondrial membrane permeability transition, nuclear disintegration and DNA fragmentation, indicators of programmed cell death (PCD) all showed significant increases under osmotic stress conditions in the Delta Fphog1 mutant in comparison to the wild type strain. These results suggest that an important function of Fphog1 is attenuating apoptotic phenotypes under salt and sorbitol stressors.
Authors:
Attila L Adám; Gábor Kohut; László Hornok
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of basic microbiology     Volume:  48     ISSN:  0233-111X     ISO Abbreviation:  J. Basic Microbiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-03     Completed Date:  2008-07-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8503885     Medline TA:  J Basic Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  151-9     Citation Subset:  IM    
Affiliation:
Agricultural Biotechnology Center, Mycology Group of the Hungarian Academy of Sciences, Institute of Plant Protection, Szent István University H-2103 Gödöllo, Páter K. u.1, Hungary. aadam@abc.hu
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
DNA Fragmentation
DNA, Fungal / isolation & purification
Fusarium / enzymology,  genetics*,  physiology*
Gene Expression Regulation, Fungal
Genes, Fungal*
Homeostasis
Hot Temperature
Hydrogen Peroxide / metabolism
Mitochondrial Membranes / physiology
Mitogen-Activated Protein Kinases / genetics,  physiology*
Osmotic Pressure
Permeability
Polymerase Chain Reaction
Reactive Oxygen Species / metabolism
Sodium Chloride / metabolism
Sorbitol / metabolism
Spores, Fungal / physiology
Transformation, Genetic
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/Reactive Oxygen Species; 50-70-4/Sorbitol; 7647-14-5/Sodium Chloride; 7722-84-1/Hydrogen Peroxide; EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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