| Foxp3+IL-17+ T cells promote development of cancer-initiating cells in colorectal cancer. | |
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MedLine Citation:
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PMID: 20952660 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The pathogenesis of CRC remains to be further understood. This study was designed to elucidate the role of Foxp3+IL-17+ T cells in the pathogenesis of CRC. Surgically removed CRC tissue was collected from 12 patients with CRC. The frequency and cytokine profile of Foxp3+IL-17+ T cells in CRC were examined by flow cytometry. Chemokine CXCL11 was examined in CRC tissue by Western blotting. Treg chemotaxis was examined in a transwell system. The effect of Foxp3+IL-17+ T cells on induction of cancer-initiating cells was examined; the latter's Akt and MAPK activities and colony formation were examined afterward. Abundant Foxp3+IL-17+ T cells were detected in CRC tissue that expresses high levels of TGF-β, CXCR3, CCR6, and RORγt. High levels of CXCL11 were detected in CRC tissue-derived CD68+ cells, which had a strong chemotactic effect on Foxp3+ Tregs. Hypoxia induced the expression of IL-17 in Foxp3+ Tregs; Foxp3+IL-17+ T cells were capable of inducing CRC-associated cell markers in BMMo and drove the cells to be cancer-initiating cells. High levels of phosphorylated Akt and MAPK were detected in the induced cancer-initiation cells; the latter has the capability to form a colony. CRC tissue-derived Foxp3+IL-17+ cells have the capacity to induce cancer-initiating cells. |
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Authors:
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Shaobo Yang; Binquan Wang; Chunying Guan; Benyan Wu; Changhao Cai; Mengwei Wang; Binsheng Zhang; Tao Liu; Pingchang Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-15 |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 89 ISSN: 1938-3673 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 85-91 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology (South Building), China PLA General Hospital, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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