| FoxO3a mediates transforming growth factor-beta1-induced apoptosis in FaO rat hepatoma cells. | |
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MedLine Citation:
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PMID: 18959820 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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FoxO3a is a member of the forkhead box class O (FoxO) transcription factor family and an important regulator of apoptosis. This work aimed to elucidate the involvement of FoxO3a in transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in FaO rat hepatoma cells. TGF-beta1 caused a time-dependent activation of FoxO3a and a subsequent increase in FoxO response-element-containing luciferase reporter activity, which was Akt-sensitive. The FaO cells stably transfected with a wild type FoxO3a were more susceptible to the formation of apoptotic bodies, populations of sub-G1 apoptotic cells, and collapse of the mitochondrial-membrane potential triggered by TGF-beta1. In contrast, transfection with small-interfering RNA (siRNA) oligonucleotide specific for FoxO3a significantly inhibited caspase activation in FaO cells treated with TGF-beta1. It thus appears that FoxO3a plays a crucial mediatory role in the TGF-beta1 signaling pathway leading to apoptosis. |
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Authors:
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Byung-Chul Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: BMB reports Volume: 41 ISSN: 1976-6696 ISO Abbreviation: BMB Rep Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-30 Completed Date: 2009-02-09 Revised Date: 2009-04-27 |
Medline Journal Info:
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Nlm Unique ID: 101465334 Medline TA: BMB Rep Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 728-32 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Korea. bckim@kangwon.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Carcinoma, Hepatocellular / metabolism*, pathology* Forkhead Transcription Factors / metabolism* Liver Neoplasms / metabolism*, pathology* Phosphorylation / drug effects Rats Transcriptional Activation / drug effects Transforming Growth Factor beta1 / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Forkhead Transcription Factors; 0/Foxo3a protein, rat; 0/Transforming Growth Factor beta1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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