| Fowlpox virus recombinants expressing the envelope glycoprotein of an avian reticuloendotheliosis retrovirus induce neutralizing antibodies and reduce viremia in chickens. | |
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MedLine Citation:
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PMID: 8388488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eight stable fowlpox virus (FPV) recombinants which express the envelope glycoprotein of the spleen necrosis virus (SNV) strain of reticuloendotheliosis virus (REV), an avian retrovirus, were constructed. These recombinants differ in the genomic location of the inserted genes, in the orientation of the insert relative to flanking viral sequences, and in the promoter used to drive expression of the env gene. Of these variables, promoter strength seems to be the most crucial. The P7.5 promoter of vaccinia virus, which is commonly used in the construction of both vaccinia virus and FPV recombinants, resulted in lower levels of expression of the envelope antigen in infected chicken cells compared with a strong synthetic promoter, as determined by immunofluorescence and enzyme-linked immunosorbent assay. Two peptides encoded by the env gene, the 21-kDa transmembrane peptide and a 62-kDa precursor, were detected by immunoprecipitation of labeled proteins from cells infected with recombinant FPVs, using monoclonal antibodies against REV. These peptides comigrated with those precipitated from REV-infected cells. One of the recombinants (f29R-SNenv) was used for vaccination of 1-day-old chickens. Vaccinated chicks developed neutralizing antibodies to SNV more rapidly than did unvaccinated controls following SNV challenge and were protected against both viremia and the SNV-induced runting syndrome. |
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Authors:
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J G Calvert; K Nazerian; R L Witter; N Yanagida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of virology Volume: 67 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1993 Jun |
Date Detail:
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Created Date: 1993-06-21 Completed Date: 1993-06-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3069-76 Citation Subset: IM; X |
Affiliation:
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USDA/ARS Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Viral Antibody Formation Body Weight Chickens Fluorescent Antibody Technique Fowlpox virus / genetics Gene Products, env / genetics, immunology* Neutralization Tests Precipitin Tests Recombinant Proteins / immunology Recombination, Genetic Reticuloendotheliosis virus / genetics, immunology* Retroviridae Proteins / genetics, immunology* Tumor Virus Infections / immunology*, prevention & control Vaccination Weight Gain |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Gene Products, env; 0/Recombinant Proteins; 0/Retroviridae Proteins; 0/avian retrovirus proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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