Document Detail


Fowlpox virus recombinants expressing the envelope glycoprotein of an avian reticuloendotheliosis retrovirus induce neutralizing antibodies and reduce viremia in chickens.
MedLine Citation:
PMID:  8388488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eight stable fowlpox virus (FPV) recombinants which express the envelope glycoprotein of the spleen necrosis virus (SNV) strain of reticuloendotheliosis virus (REV), an avian retrovirus, were constructed. These recombinants differ in the genomic location of the inserted genes, in the orientation of the insert relative to flanking viral sequences, and in the promoter used to drive expression of the env gene. Of these variables, promoter strength seems to be the most crucial. The P7.5 promoter of vaccinia virus, which is commonly used in the construction of both vaccinia virus and FPV recombinants, resulted in lower levels of expression of the envelope antigen in infected chicken cells compared with a strong synthetic promoter, as determined by immunofluorescence and enzyme-linked immunosorbent assay. Two peptides encoded by the env gene, the 21-kDa transmembrane peptide and a 62-kDa precursor, were detected by immunoprecipitation of labeled proteins from cells infected with recombinant FPVs, using monoclonal antibodies against REV. These peptides comigrated with those precipitated from REV-infected cells. One of the recombinants (f29R-SNenv) was used for vaccination of 1-day-old chickens. Vaccinated chicks developed neutralizing antibodies to SNV more rapidly than did unvaccinated controls following SNV challenge and were protected against both viremia and the SNV-induced runting syndrome.
Authors:
J G Calvert; K Nazerian; R L Witter; N Yanagida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  67     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-06-21     Completed Date:  1993-06-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3069-76     Citation Subset:  IM; X    
Affiliation:
USDA/ARS Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Viral
Antibody Formation
Body Weight
Chickens
Fluorescent Antibody Technique
Fowlpox virus / genetics
Gene Products, env / genetics,  immunology*
Neutralization Tests
Precipitin Tests
Recombinant Proteins / immunology
Recombination, Genetic
Reticuloendotheliosis virus / genetics,  immunology*
Retroviridae Proteins / genetics,  immunology*
Tumor Virus Infections / immunology*,  prevention & control
Vaccination
Weight Gain
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Gene Products, env; 0/Recombinant Proteins; 0/Retroviridae Proteins; 0/avian retrovirus proteins
Comments/Corrections

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