Document Detail


Fourteen-year experience of acetylcholinesterase staining for rectal mucosal biopsy in neonatal Hirschsprung's disease.
MedLine Citation:
PMID:  11528605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/PURPOSE: Acetylcholinesterase (AChE) staining of rectal mucosal biopsy specimens is the most important and popular examination for making a definite diagnosis of Hirschsprung's disease. This examination often is performed for patients with constipation in the daily clinic. The results of this examination are reflected immediately in the treatment. However, the authors sometimes encountered difficult cases to diagnose, especially in neonates. Therefore, a retrospective investigation was conducted on the benefits and problems of AChE staining of rectal mucosal biopsy specimens in neonates. METHODS: The authors encountered 459 cases (91 neonates) of suspected Hirschsprung's disease, clinically, from April 1986 to March 2000. Mucosal specimens were taken by punch biopsies. Samples were stained by the modified Karnovsky Roots method using rubeanic acid as an amplifier and immediately examined with a light microscope. These results were collected and assessed mainly on neonatal cases. The authors also analyzed the 104 cases of Hirschsprung's disease diagnosed in patients less than 1 year of age to evaluate the relationship between the grade of proliferation of AChE positive fiber and age. RESULTS: Forty-one neonatal cases of Hirschsprung's disease were diagnosed based on the findings of AChE staining. A definite diagnosis of Hirschsprung's disease was confirmed based on the pathologic findings of operative samples. Forty-eight cases that were diagnosed as normal included 4 cases that turned out to be false-negative (3 Hirschspurung's disease cases and 1 case of an allied disorder of Hirschsprung's disease). There were no major complications in mucosal punch biopsy. In the cases of Hirschsprung's disease diagnosed in a patient less than 1 year of age, the grade of AChE-positive fiber tended to increase with the aging of patients. CONCLUSIONS: The specificity of AChE staining was high (100%), but its sensitivity was slightly low (91%). Careful long-term follow-up is required for any cases diagnosed as normal. Mucosal biopsies should be repeated in cases of persistent clinical symptoms.
Authors:
M Nakao; S Suita; T Taguchi; R Hirose; Y Shima
Related Documents :
2404215 - Charcot-marie-tooth disease and nephropathy in a mother and daughter with a review of t...
24704725 - Reversible blindness in cryptococcal meningitis with normal intracranial pressure: case...
21399245 - Surgical treatment in a case of giant scrotal lymphedema.
20427925 - Numb chin syndrome: a case report and review of the literature.
11565085 - Clostridium difficile small bowel enteritis occurring after total colectomy.
22022115 - Laparoscopic cholecystectomy and appendicectomy in situs inversus totalis: a case repor...
Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  36     ISSN:  0022-3468     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-08-30     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1357-63     Citation Subset:  IM    
Copyright Information:
Copyright 2001 by W.B. Saunders Company.
Affiliation:
Departments of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / diagnostic use*
Biopsy, Needle
Female
Hirschsprung Disease / diagnosis,  pathology*
Humans
Immunohistochemistry
Indicators and Reagents
Infant, Newborn
Intestinal Mucosa / pathology*
Male
Rectum / pathology
Retrospective Studies
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Indicators and Reagents; EC 3.1.1.7/Acetylcholinesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Multidisciplinary behavioral treatment of defecation problems: a controlled study in children with a...
Next Document:  Solo-RBT: a new instrument for rectal suction biopsies in the diagnosis of Hirschsprung's disease.