Document Detail

Fourier transform infrared spectroscopic imaging of cardiac tissue to detect collagen deposition after myocardial infarction.
MedLine Citation:
PMID:  22612137     Owner:  NLM     Status:  MEDLINE    
Myocardial infarction often leads to an increase in deposition of fibrillar collagen. Detection and characterization of this cardiac fibrosis is of great interest to investigators and clinicians. Motivated by the significant limitations of conventional staining techniques to visualize collagen deposition in cardiac tissue sections, we have developed a Fourier transform infrared imaging spectroscopy (FT-IRIS) methodology for collagen assessment. The infrared absorbance band centered at 1338  cm(-1), which arises from collagen amino acid side chain vibrations, was used to map collagen deposition across heart tissue sections of a rat model of myocardial infarction, and was compared to conventional staining techniques. Comparison of the size of the collagen scar in heart tissue sections as measured with this methodology and that of trichrome staining showed a strong correlation (R=0.93). A Pearson correlation model between local intensity values in FT-IRIS and immuno-histochemical staining of collagen type I also showed a strong correlation (R=0.86). We demonstrate that FT-IRIS methodology can be utilized to visualize cardiac collagen deposition. In addition, given that vibrational spectroscopic data on proteins reflect molecular features, it also has the potential to provide additional information about the molecular structure of cardiac extracellular matrix proteins and their alterations.
Rabee Cheheltani; Jenna M Rosano; Bin Wang; Abdel Karim Sabri; Nancy Pleshko; Mohammad F Kiani
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  17     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-22     Completed Date:  2012-09-28     Revised Date:  2013-06-24    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  056014     Citation Subset:  IM    
Temple University, Department of Mechanical Engineering, Philadelphia, PA, USA.
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MeSH Terms
Biological Markers / analysis
Collagen / analysis*
Myocardial Infarction / metabolism*
Myocardium / metabolism*
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Spectroscopy, Fourier Transform Infrared / methods*
Reg. No./Substance:
0/Biological Markers; 9007-34-5/Collagen

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