Document Detail


Forward genetics in mammalian cells: functional approaches to gene discovery.
MedLine Citation:
PMID:  10469846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Definitive proof of function in biological systems requires genetic analysis. Only when the loss of a particular protein corresponds to the loss of a specific function can one be sure that the protein truly affects the function. Changing the pattern of gene expression through random mutagenesis or by introducing expression libraries, followed by selection of mutant or variant cells and identification of a missing or overexpressed protein, has the power to reveal or confirm the roles of specific components of signaling pathways and to provide mutant cell lines and cDNA reagents to be used in defining detailed mechanisms through structure-function analyses. These examples of forward genetics contrast with reverse genetic approaches, where the function of a known gene product is explored by knockout or replacement. Here we review a broad range of techniques that have been used to alter gene expression randomly in mammalian cells, with examples of specific discoveries that have resulted from these applications of forward genetics.
Authors:
G R Stark; A V Gudkov
Related Documents :
19943896 - Who is the bosr around here anyway?
7583156 - Molecular biology in the intensive care unit: a framework for interpretation.
12072916 - In silico analyses of mouse inner-ear transcripts.
2184746 - Biology and clinical aspects of the hematopoietic growth factors.
18439596 - Differences in gene expression of granulosa cells from women undergoing controlled ovar...
15703836 - Hla genes and other candidate genes involved in susceptibility for (pre)neoplastic cerv...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Human molecular genetics     Volume:  8     ISSN:  0964-6906     ISO Abbreviation:  Hum. Mol. Genet.     Publication Date:  1999  
Date Detail:
Created Date:  2000-07-13     Completed Date:  2000-07-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9208958     Medline TA:  Hum Mol Genet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1925-38     Citation Subset:  IM    
Affiliation:
Lerner Research Institute, The Cleveland Clinic Foundation, OH 44195, USA, starkg@ccf.org
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cloning, Molecular / methods*
Gene Expression / genetics
Genes* / genetics,  physiology
Genes, Dominant / genetics
Genes, Recessive / genetics
Mutagenesis* / genetics
Selection, Genetic
Transfection
Grant Support
ID/Acronym/Agency:
CA60730/CA/NCI NIH HHS; CA62220/CA/NCI NIH HHS; CA75179/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular genetics of the Finnish disease heritage.
Next Document:  Genetics of HIV-1 infection: chemokine receptor CCR5 polymorphism and its consequences.