Document Detail


Forward chemical screening of small RNA pathways.
MedLine Citation:
PMID:  24306865     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
RNA silencing is a mechanism of gene expression regulation mediated by short noncoding RNAs called small RNAs. Small RNAs can suppress gene expression transcriptionally or posttranscriptionally by base pairing to their targets. In plants, they are involved in a diverse range of pathways and processes. Forward genetic screens have led to the identification of many components in small RNA biogenesis and functional pathways. However, it cannot identify essential or functionally redundant genes. Forward chemical screens can overcome these limitations by targeting multiple redundant proteins within a family and by giving the user temporal control of the application of the chemical. Here, we describe a method to quickly screen chemicals that perturb small RNA pathways using Arabidopsis thaliana reporter lines in a 96-well format.
Authors:
Yifan Lii; Hailing Jin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1056     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2014  
Date Detail:
Created Date:  2013-12-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  95-101     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology and Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, CA, USA.
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