Document Detail


Forty-hour preservation of the rabbit heart: optimal osmolarity, [Mg2+], and pH of a modified UW solution.
MedLine Citation:
PMID:  8037563     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The University of Wisconsin solution modified with 2,3-butanedione monoxime and calcium experimentally extends the limits of ischemic preservation of the heart. This study evaluates other characteristics of this modified solution that may further enhance preservation: osmolarity, Mg2+ concentration ([Mg2+]), and pH. Rabbit hearts were flushed with the modified University of Wisconsin solution and stored for 40 hours at 4 degrees C. Maximal left ventricular developed pressure (LVDP), left ventricular end-diastolic volume (LVEDV), maximum rate of increase of left ventricular pressure (dP/dt), heart rate, and coronary flow were measured during 60 minutes of isolated crystalloid reperfusion with an isovolumic left ventricular balloon at constant end-diastolic pressure. Creatine kinase release and myocardial adenine nucleotide content were measured at completion of reperfusion. Solution osmolarity was tested at 357, 327, 297, and 277 mOsm/L by reducing K+, Na+, and lactobionate concentrations. [Mg2+] was assessed at 5 and 16 mmol/L. Solution pH was studied at 7.0, 7.4, and 7.8. A control group of hearts was flushed and immediately reperfused to establish baseline function. Hearts stored in either hypertonic (357 mOsm/L) or hypotonic (277 mOsm/L) solutions functioned poorly, reaching 58% and 50% of control LVDP (p < 0.001), 49% (p < 0.01), and 58% (p = not significant) of LVEDV, 56% and 49% of +dP/dt (p < 0.001), respectively, and released substantially more creatine kinase (p < 0.001 versus control).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J C Stringham; K L Paulsen; J H Southard; R M Mentzer; F O Belzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  58     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-18     Completed Date:  1994-08-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-13     Citation Subset:  AIM; IM    
Affiliation:
Division of Cardiothoracic Surgery, University of Wisconsin Hospital and Clinics, Madison 53792.
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MeSH Terms
Descriptor/Qualifier:
Adenosine / chemistry
Allopurinol / chemistry
Animals
Calcium*
Cardioplegic Solutions / chemistry*
Chromogenic Compounds
Creatine Kinase / metabolism
Diacetyl / analogs & derivatives*
Glutathione / chemistry
Heart Transplantation* / physiology
Hydrogen-Ion Concentration
Insulin / chemistry
Magnesium*
Myocardial Contraction / drug effects
Myocardium / enzymology
Organ Preservation*
Organ Preservation Solutions*
Osmolar Concentration
Rabbits
Raffinose / chemistry
Time Factors
Ventricular Function / drug effects
Chemical
Reg. No./Substance:
0/Cardioplegic Solutions; 0/Chromogenic Compounds; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 431-03-8/Diacetyl; 512-69-6/Raffinose; 57-71-6/diacetylmonoxime; 58-61-7/Adenosine; 70-18-8/Glutathione; 7439-95-4/Magnesium; 7440-70-2/Calcium; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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