Document Detail

Forskolin-stimulated adenylylcyclase activity: a marker to assess islet cell viability following cold storage in different solutions and to predict islet cell function following transplantation.
MedLine Citation:
PMID:  10478718     Owner:  NLM     Status:  MEDLINE    
For clinical islet cell transplantation, short-term storage of islet cells is likely to be necessary, and it is imperative that the islet cells be kept as viable as possible during the period. However, there are little data on which preservative solutions are most suitable for the storage of islet cells after isolations or before transplantation. To estimate islet cell viability and transplantation success rate in the present study, adenylylcyclase activity was measured with a rapid new fluorometric assay in rat islet cells prior to transplantation, because cAMP plays an essential role in determining islet beta-cell viability and responsiveness to various hormonal stimuli. Adenylylcyclase activity was measured in islet cells stored for different periods of time 0, 3, 16, 24, 48, 96 h) and in different preservative solutions. Approximately 1,000 islet cells from each preservation group using University of Wisconsin (UW) solution were transplanted to streptozotocin-induced diabetes mellitus (DM) rats. Transplant success was evaluated by measuring blood glucose levels. Preoperative adenylylcyclase activity was compared with posttransplant islet cell function. The adenylylcyclase activity of UW solution was significantly higher than that of Euro-Collins solution and lactate-Ringer's solution through the different preservation time periods. Preoperative adenylylcyclase activity correlated well with posttransplant islet cell function in a rat model of DM. We conclude that adenylylcyclase activity can be used as a marker to assess islet cell viability as well as differences in preservation media and may predict islet cell transplant success.
S Kanazawa; T Tsunoda; M Murakami; S Iwamoto; M Kimoto; E Onuma; T Majima; Y Yamamoto
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell transplantation     Volume:  8     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:    1999 Jul-Aug
Date Detail:
Created Date:  1999-10-20     Completed Date:  1999-10-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  383-8     Citation Subset:  IM    
Department of Surgery, Mitsugi General Hospital, Hiroshima, Mitsugi-gun, Japan.
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MeSH Terms
Adenylate Cyclase / metabolism*
Biological Markers
Blood Glucose
Cell Survival / physiology
Cold Temperature*
Cyclic AMP / metabolism
Diabetes Mellitus, Experimental / metabolism,  surgery
Enzyme Activation / drug effects,  physiology
Forskolin / pharmacology*
Graft Survival / physiology*
Hypertonic Solutions / pharmacology
Islets of Langerhans / cytology,  enzymology
Islets of Langerhans Transplantation*
Isotonic Solutions / pharmacology
Organ Preservation Solutions / pharmacology
Rats, Inbred Lew
Time Factors
Reg. No./Substance:
0/Biological Markers; 0/Blood Glucose; 0/Euro-Collins' solution; 0/Hypertonic Solutions; 0/Isotonic Solutions; 0/Organ Preservation Solutions; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 8022-63-7/Ringer's lactate; EC Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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