Document Detail


Formulation and in vitro characterization of composite biodegradable magnetic nanoparticles for magnetically guided cell delivery.
MedLine Citation:
PMID:  22274555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Cells modified with magnetically responsive nanoparticles (MNP) can provide the basis for novel targeted therapeutic strategies. However, improvements are required in the MNP design and cell treatment protocols to provide adequate magnetic properties in balance with acceptable cell viability and function. This study focused on select variables controlling the uptake and cell compatibility of biodegradable polymer-based MNP in cultured endothelial cells.
METHODS: Fluorescent-labeled MNP were formed using magnetite and polylactide as structural components. Their magnetically driven sedimentation and uptake were studied fluorimetrically relative to cell viability in comparison to non-magnetic control conditions. The utility of surface-activated MNP forming affinity complexes with replication-deficient adenovirus (Ad) for transduction achieved concomitantly with magnetic cell loading was examined using the green fluorescent protein reporter.
RESULTS: A high-gradient magnetic field was essential for sedimentation and cell binding of albumin-stabilized MNP, the latter being rate-limiting in the MNP loading process. Cell loading up to 160 pg iron oxide per cell was achievable with cell viability >90%. Magnetically driven uptake of MNP-Ad complexes can provide high levels of transgene expression potentially useful for a combined cell/gene therapy.
CONCLUSIONS: Magnetically responsive endothelial cells for targeted delivery applications can be obtained rapidly and efficiently using composite biodegradable MNP.
Authors:
Michael Chorny; Ivan S Alferiev; Ilia Fishbein; Jillian E Tengood; Zoë Folchman-Wagner; Scott P Forbes; Robert J Levy
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-01-25
Journal Detail:
Title:  Pharmaceutical research     Volume:  29     ISSN:  1573-904X     ISO Abbreviation:  Pharm. Res.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-24     Completed Date:  2012-09-13     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1232-41     Citation Subset:  IM    
Affiliation:
Division of Cardiology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA. chorny@email.chop.edu
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MeSH Terms
Descriptor/Qualifier:
Absorbable Implants
Animals
Cattle
Cell Survival
Cells, Cultured
Chemistry, Pharmaceutical
Drug Delivery Systems*
Drug Stability
Endothelial Cells / metabolism*
Ferumoxytol / chemistry
Fluorescent Dyes / chemistry
Gene Transfer Techniques
Kinetics
Magnetics*
Molecular Structure
Nanoparticles*
Particle Size
Polyesters / chemistry
Surface-Active Agents / chemistry
Grant Support
ID/Acronym/Agency:
HL72108/HL/NHLBI NIH HHS; HL94816/HL/NHLBI NIH HHS; R01 HL072108/HL/NHLBI NIH HHS; R01 HL072108-07/HL/NHLBI NIH HHS; R21 HL094816-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Polyesters; 0/Surface-Active Agents; 1317-61-9/Ferumoxytol; 26969-66-4/poly(lactide)
Comments/Corrections

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