Document Detail


Formula Diet is Effective for the Reduction and Differentiation of Visceral Adipose Tissue in Zucker Fatty Rats.
MedLine Citation:
PMID:  22104175     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aim: Formula diet (FD), which is used as a tool for calorie restriction, has beneficial effects on metabolic disorders in obese patients; however, the molecular mechanism is not fully understood.Methods: Sixteen-week-old male Zucker Diabetic Fatty (ZDF) rats were divided into 3 groups (n= 10 each): calorie-controlled low-fat diet [Control; 75 kcal/day, protein : fat : carbohydrate (P:F:C)=25:15: 60], calorie-restricted low-fat diet (CR-LFD; 56 kcal/day, P:F:C=25:15:60), and calorie-restricted FD (CR-FD; 56 kcal/day, P:F:C=50:14:36) group, and fed each diet for 4 weeks. Before the study, baseline data were obtained in 10 rats. After 4 weeks, body weight and epididymal fat weight were measured, and blood samples, mesenteric and subcutaneous adipose tissues were collected for analyses. Messenger RNA expression was evaluated by real-time PCR, and protein expression by Western blotting.Results: The decrease in epididymal fat weight was significantly greater in the CR-FD group than in control and CR-LFD groups, although changes in body weight were not different among groups. The decrease in fasting plasma glucose and increase in plasma adiponectin were greater in the CR-FD group than in the control group, but not in the CR-LFD group. The decrease in triglyceride and increase in HDL-cholesterol were greatest in the CR-FD group. Both mRNA and protein of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α, adiponectin, lipoprotein lipase and PPARγ were overexpressed in the CR-FD group, especially in mesenteric adipose tissue.Conclusions: FD may have beneficial effects on abdominal obesity and metabolic disorders by reducing visceral fat and improving glucose and lipid profiles, possibly through modulating adipose tissue function.Key words; Formula diet, High protein diet, Low carbohydrate diet, Obesity, Zucker diabetic fatty rat.
Authors:
Takashi Yamaguchi; Yoh Miyashita; Atsuhito Saiki; Fusako Watanabe; Hitoshi Watanabe; Kohji Shirai
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-21
Journal Detail:
Title:  Journal of atherosclerosis and thrombosis     Volume:  -     ISSN:  1880-3873     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506298     Medline TA:  J Atheroscler Thromb     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Center of Diabetes, Endocrine and Metabolism, Toho University Sakura Medical Center.
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