Document Detail

Forms of Selenium Affect its Transport, Uptake and Glutathione Peroxidase Activity in the Caco-2 Cell Model.
MedLine Citation:
PMID:  22451375     Owner:  NLM     Status:  Publisher    
The experiment was designed to investigate the effect of selenium (Se) chemical forms (sodium selenite, selenium nanoparticle [nano-Se] and selenomethionine) on the transport, uptake and glutathione peroxidase (GSH-Px) activity in the Caco-2 cell model. The transport and uptake of different forms of Se (0.1 μmol l(-1)) across the Caco-2 cell monolayer were carried out in two directions (apical [AP] to basolateral [BL] and BL to AP) for 2 h, respectively, and the apparent permeability coefficient (P (app)), transport efficiency and uptake efficiency were all calculated. In the present study, the transport and uptake of three forms of Se were time-dependent both in AP to BL and BL to AP directions. By the end of 2 h, the transport efficiencies of selenomethionine and nano-Se were higher than that of sodium selenite (P < 0.05). The highest uptake efficiency (P < 0.05) was observed in cells treated with nano-Se and significant difference (P < 0.05) was also observed between the cells incubated with sodium selenite and selenomethionine. As for the P (app), sodium selenite (P < 0.05) had the lowest values compared with that of selenomethionine and nano-Se, in both AP-BL and BL-AP. However, no significant differences were observed in GSH-Px activities. These results indicated that the efficiency of Se in the Caco-2 cells varied with its chemical forms, which might be associated with the differences in Se transport and uptake.
Yanbo Wang; Linglin Fu
Related Documents :
22872085 - Dally and notum regulate the switch between low and high level hedgehog pathway signall...
7631535 - Comparison of thinprep preparations with conventional cervicovaginal smears. practical ...
21205075 - Cellular responses to chlorin-based photosensitizer dh-ii-24 under darkness in human ga...
6998555 - Diffuse large cell lymphomas (reticulum cell sarcomas, histiocytic lymphomas). correlat...
488125 - Cylindrical laminated bodies in nickel-subsulphide-induced rhabdomyosarcoma in rabbits.
24472895 - On-chip self-assembly of cell embedded microstructures to vascular-like microtubes.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-28
Journal Detail:
Title:  Biological trace element research     Volume:  -     ISSN:  1559-0720     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Key Laboratory of Food Safety of Zhejiang Province, Food Quality and Safety Department, Zhejiang Gongshang University, 149 Jiaogong Road, Hangzhou, 310035, Zhejiang province, China,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A test of Rescorla and Wagner's (1972) prediction of nonlinear effects in contingency learning.
Next Document:  Prenatal Exposure to Arsenic and Its Effects on Fetal Development in the General Population of Dalia...