Document Detail


FORMING SELF-ASSEMBLED CELL ARRAYS AND MEASURING THE OXYGEN CONSUMPTION RATE OF A SINGLE LIVE CELL.
MedLine Citation:
PMID:  20694048     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We report a method for forming arrays of live single cells on a chip using polymer micro-traps made of SU8. We have studied the toxicity of the microfabricated structures and the associated environment for two cell lines. We also report a method for measuring the oxygen consumption rate of a single cell using optical interrogation of molecular oxygen sensors placed in micromachined micro-wells by temporarily sealing the cells in the micro-traps. The new techniques presented here add to the collection of tools available for performing "single-cell" biology. A single-cell self-assembly yield of 61% was achieved with oxygen draw down rates of 0.83, 0.82, and 0.71 fmol/minute on three isolated live A549 cells.
Authors:
James R Etzkorn; Sarah C McQuaide; Judy B Anderson; Deirdre R Meldrum; Babak A Parviz
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Digest of technical papers / International Conference on Solid-State Sensors, Actuators and Microsystems. International Conference on Solid-State Sensors, Actuators, and Microsystems     Volume:  2009     ISSN:  -     ISO Abbreviation:  -     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2010-8-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101525070     Medline TA:  Dig Tech Papers     Country:  -    
Other Details:
Languages:  ENG     Pagination:  2374-2377     Citation Subset:  -    
Affiliation:
Electrical Engineering, University of Washington, Seattle, WA, USA.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
P50 HG002360-11//NHGRI NIH HHS

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