| FORMING SELF-ASSEMBLED CELL ARRAYS AND MEASURING THE OXYGEN CONSUMPTION RATE OF A SINGLE LIVE CELL. | |
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MedLine Citation:
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PMID: 20694048 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We report a method for forming arrays of live single cells on a chip using polymer micro-traps made of SU8. We have studied the toxicity of the microfabricated structures and the associated environment for two cell lines. We also report a method for measuring the oxygen consumption rate of a single cell using optical interrogation of molecular oxygen sensors placed in micromachined micro-wells by temporarily sealing the cells in the micro-traps. The new techniques presented here add to the collection of tools available for performing "single-cell" biology. A single-cell self-assembly yield of 61% was achieved with oxygen draw down rates of 0.83, 0.82, and 0.71 fmol/minute on three isolated live A549 cells. |
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Authors:
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James R Etzkorn; Sarah C McQuaide; Judy B Anderson; Deirdre R Meldrum; Babak A Parviz |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Digest of technical papers / International Conference on Solid-State Sensors, Actuators and Microsystems. International Conference on Solid-State Sensors, Actuators, and Microsystems Volume: 2009 ISSN: - ISO Abbreviation: - Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2010-8-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101525070 Medline TA: Dig Tech Papers Country: - |
Other Details:
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Languages: ENG Pagination: 2374-2377 Citation Subset: - |
Affiliation:
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Electrical Engineering, University of Washington, Seattle, WA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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P50 HG002360-11//NHGRI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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