Document Detail

Forming double layer-encapsulated quantum dots for bio-imaging and cell targeting.
MedLine Citation:
PMID:  23314757     Owner:  NLM     Status:  Publisher    
We report a simple and effective approach for the preparation of double layer-encapsulated quantum dots (DL-Qdots) composed of alkyl-capping ligands to interdigitate with hydrophobic, protective agents on the surface of AgInS(2)/ZnS quantum dots (Qdots), which allow phase transfer of hydrophobic Qdots from the organic phase into the aqueous phase. The alkyl-capping ligands consist of a hydrophobic, aliphatic chain and different functional terminal groups (e.g., carboxyl, amine, hydroxyl, and thiol groups) that can serve as reactive sites to chemically couple with other materials. The resulting DL-Qdots bearing various functional groups retain good fluorescence properties and show excellent solubility as well as stability over a range of pH in the aqueous phase. Cytotoxicity studies of DL-Qdots bearing carboxyl groups (DL-Qdots-COOH) were carried out against human cervical (HeLa) cancer cells to elicit no apparent toxicity even at high concentrations of 300 μg mL(-1) and 24 h of incubation. To demonstrate their potential biomedical application, DL-Qdots-COOH were further conjugated with folate for staining in HeLa, human liver carcinoma (HepG2), and human breast (MCF-7) cancer cells. Confocal imaging characterization revealed that folate-conjugated DL-Qdots could target most specifically and effectively HeLa cells via folate receptor-mediated targeted delivery compared to HepG2 and MCF-7 cells. The generality and simplicity of this newly developed strategy can possibly be extended to a large variety of hydrophobic Qdots and nanocrystals whose surface protective agents have a long aliphatic chain.
Mochamad Zakki Fahmi; Jia-Yaw Chang
Related Documents :
23646617 - Comparative bio-effects of sio2/gd2o3 nanoparticles depending on their core-shell struc...
24825397 - Androgen-dependent sertoli cell tight junction remodeling is mediated by multiple tight...
24867957 - N-glycosylation is required for matriptase-2 autoactivation and ectodomain shedding.
23686647 - Multidimensional control of cell structural robustness.
18397337 - Cell proliferation, apoptosis and mitochondrial damage in rat b50 neuronal cells after ...
12488537 - Breast cancer resistance protein (bcrp/abcg2) induces cellular resistance to hiv-1 nucl...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-14
Journal Detail:
Title:  Nanoscale     Volume:  -     ISSN:  2040-3372     ISO Abbreviation:  Nanoscale     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Chemical Engineering, National Taiwan University of Science and Technology, Section 4, #43, Keelung Road, Taipei 106, Taiwan, ROC.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Anticancer effects of O-desmethylangolensin are mediated through cell cycle arrest at the G2/M phase...
Next Document:  Depression in heart failure.