Document Detail


Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.
MedLine Citation:
PMID:  22760034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An on-plate testing method using formic acid was evaluated on the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry system using 90 yeast and 78 Corynebacterium species isolates, and 95.6 and 81.1% of yeast and 96.1 and 92.3% of Corynebacterium isolates were correctly identified to the genus and species levels, respectively. The on-plate method using formic acid yielded identification percentages similar to those for the conventional but more laborious tube-based extraction.
Authors:
Elitza S Theel; Bryan H Schmitt; Leslie Hall; Scott A Cunningham; Robert C Walchak; Robin Patel; Nancy L Wengenack
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2012-07-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-16     Completed Date:  2013-01-03     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3093-5     Citation Subset:  IM    
Affiliation:
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
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MeSH Terms
Descriptor/Qualifier:
Corynebacterium / chemistry,  isolation & purification*
Formates / diagnostic use*
Humans
Microbiological Techniques / methods*
Specimen Handling / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Yeasts / chemistry,  isolation & purification*
Chemical
Reg. No./Substance:
0/Formates; 64-18-6/formic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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