| Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. | |
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MedLine Citation:
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PMID: 22760034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An on-plate testing method using formic acid was evaluated on the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry system using 90 yeast and 78 Corynebacterium species isolates, and 95.6 and 81.1% of yeast and 96.1 and 92.3% of Corynebacterium isolates were correctly identified to the genus and species levels, respectively. The on-plate method using formic acid yielded identification percentages similar to those for the conventional but more laborious tube-based extraction. |
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Authors:
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Elitza S Theel; Bryan H Schmitt; Leslie Hall; Scott A Cunningham; Robert C Walchak; Robin Patel; Nancy L Wengenack |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2012-07-03 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 50 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-08-16 Completed Date: 2013-01-03 Revised Date: 2013-04-15 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3093-5 Citation Subset: IM |
Affiliation:
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Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Corynebacterium
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chemistry,
isolation & purification* Formates / diagnostic use* Humans Microbiological Techniques / methods* Specimen Handling / methods* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Yeasts / chemistry, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Formates; 64-18-6/formic acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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