Document Detail

Formation of urso- and ursodeoxy-cholic acids from primary bile acids by a Clostridium limosum soil isolate.
MedLine Citation:
PMID:  6512414     Owner:  NLM     Status:  MEDLINE    
A gram-positive, rod-shaped anaerobe (isolate F-14) was isolated from soil. This organism was identified by cellular morphology as well as by fermentative and biochemical data as Clostridium limosum. Isolate F-14 formed ursocholic acid (UC) and 7-ketodeoxycholic acid (7-KDC) from cholic acid (CA), and ursodeoxycholic acid (UDC) and 7-ketolithocholic acid (7-KLC) from chenodeoxycholic acid (CDC) in whole cell cultures, but did not transform deoxycholic acid (DC). No hydrolysis or transformation occurred when either taurine- or glycine-conjugated bile acids were incubated with F-14. The type stain of Clostridium limosum (American Type Culture Collection 25620) did not transform bile acids. The structures of ursocholic, ursodeoxycholic, 7-ketodeoxycholic, and 7-ketolithocholic acids were verified by mass spectroscopy and by thin-layer chromatography using Komarowsky's spray reagent. The organism transformed cholic and chenodeoxycholic acids at concentrations of 20 mM and 1 mM, respectively; higher concentrations of bile acids inhibited growth. Optimal yields of ursocholic and ursodeoxycholic acids were obtained at 9-24 hr of incubation and depended upon the substrate used. Increasing yields of 7-ketodeoxycholic and 7-ketolithocholic acids, and decreasing yields of ursocholic and ursodeoxycholic acids were observed with longer periods of incubation. Culture pH changed with time and was characterized by a small initial drop (0.2-0.4 pH units) and a subsequent increase to a pH (8.1-8.2) that was above the starting pH (7.4).(ABSTRACT TRUNCATED AT 250 WORDS)
J D Sutherland; L V Holdeman; C N Williams; I A Macdonald
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid research     Volume:  25     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-01-31     Completed Date:  1985-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1084-9     Citation Subset:  IM    
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MeSH Terms
Bile Acids and Salts / metabolism
Cholic Acid
Cholic Acids / biosynthesis*,  metabolism*
Chromatography, Thin Layer
Clostridium / metabolism*
Deoxycholic Acid / analogs & derivatives*
Soil Microbiology*
Ursodeoxycholic Acid / biosynthesis*
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cholic Acids; 128-13-2/Ursodeoxycholic Acid; 2955-27-3/ursocholic acid; 81-25-4/Cholic Acid; 83-44-3/Deoxycholic Acid

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