| Formation, toxicity and inactivation of acrolein during biotransformation of cyclophosphamide as studied in freshly isolated cells from rat liver and kidney. | |
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MedLine Citation:
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PMID: 4026579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study the formation and the effects of cyclophosphamide-derived acrolein were investigated using isolated cells from rat liver and kidney, with particular regard to the protective action of low molecular weight thiols against cellular toxicity. The results may be summarized as follows: Cyclophosphamide (CTX)-mediated toxicity to isolated cells is dependent on cytochrome P-450 activity; Loss of viability in cells incubated with cyclophosphamide is preceded by a depletion of cellular GSH; Stimulation of cellular GSH synthesis or the presence of low molecular weight thiols in the incubation medium protects against cyclophosphamide-induced toxicity; Acrolein is probably formed extracellularly as well as intracellularly and can be detoxified by thiol compounds, forming a thiochemiacetal or a thioether. |
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Authors:
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Y Ohno; K Ormstad |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of toxicology Volume: 57 ISSN: 0340-5761 ISO Abbreviation: Arch. Toxicol. Publication Date: 1985 Jun |
Date Detail:
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Created Date: 1985-09-19 Completed Date: 1985-09-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417615 Medline TA: Arch Toxicol Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 99-103 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrolein
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metabolism*,
toxicity Aldehydes / metabolism* Animals Biotransformation Chromatography, High Pressure Liquid Cyclophosphamide / metabolism*, toxicity Glutathione / metabolism Kidney / cytology, metabolism* Kinetics Liver / cytology, metabolism* Male Metabolic Detoxication, Drug Metyrapone / pharmacology Phenobarbital / pharmacology Rats Rats, Inbred Strains Sulfhydryl Compounds / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Sulfhydryl Compounds; 107-02-8/Acrolein; 50-06-6/Phenobarbital; 50-18-0/Cyclophosphamide; 54-36-4/Metyrapone; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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