Document Detail


Formation of a stable mimic of ambient particulate matter containing viable infectious Respiratory Syncytial Virus and its dry-deposition directly onto cell cultures.
MedLine Citation:
PMID:  23205519     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Epidemiological associations of worse respiratory outcomes from combined exposure to ambient particulate matter (PM) and respiratory viral infection suggest possible interactions between PM and viruses. To characterize outcomes of such exposures, we developed an in vitro mimic of the in vivo event of exposure to PM contaminated with Respiratory Syncytial Virus (RSV). Concentration of infectious RSV stocks and a particle levitation apparatus were the foundations of the methodology developed to generate specific numbers of PM mimics (PMMimics) of known composition for dry, direct deposition onto airway epithelial cell cultures. Three types of PMMimics were generated for this study: (i) carbon alone (PC), (ii) carbon and infectious RSV (PC+RSV), and (iii) aerosols consisting of RSV (ARSV). PC+RSV were stable in solution and harbored infectious RSV for up to two months. Unlike ARSV infection, PC+RSV infection was found to be dynamin dependent and to cause lysosomal rupture. Cells dosed with PMMimics comprised of RSV (ARSV), carbon (PC) or RSV and carbon (PC+RSV) responded differentially as exemplified by the secretion patterns of IL-6 and IL-8. Upon infection, and prior to lung cell death due to viral infection, regression analysis of these two mediators in response to incubation with ARSV or PC+RSV yielded higher concentrations upon infection with the latter PC+RSV and at earlier time points, than either of ARSV or PC. In conclusion, this experimental platform provides an approach to study the combined effects of PM-viral interactions and airway epithelial exposures in the pathogenesis of respiratory diseases involving inhalation of environmental agents.
Authors:
Teresita M Cruz-Sanchez; Allen E Haddrell; Tillie L Hackett; Gurpreet K Singhera; David Marchand; Ryan K Lekivetz; Anna Meredith; Derrick Horne; Darryl A Knight; Stephen F van Eeden; Tony R Bai; Richard G Hegele; Delbert R Dorsheid; George R Agnes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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