Document Detail


Formation of salivary acinar cell spheroids in vitro above a polyvinyl alcohol-coated surface.
MedLine Citation:
PMID:  18671268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue engineering of salivary glands offers the potential for future use in the treatment of patients with salivary hypofunction. Biocompatible materials that promote acinar cell aggregation and function in vitro are an essential part of salivary gland tissue engineering. In this study, rat parotid acinar cells assembled into three-dimensional aggregates above the polyvinyl alcohol (PVA)-coated surface. These aggregates developed compact acinar cell spheroids resembling in vivo physiological condition, which were different from the traditional monolayered morphology in vitro. Cells remained viable and with better functional activity in response to acetylcholine in the spheroids and could form monolayered acinar cells when they were reinoculated on tissue culture polystyrene wells. To interpret the phenomenon further, we proposed that the formation of acinar cell spheroids on the PVA is mediated by a balance between two competing forces: the interactions of cell-PVA and cell-cell. This study demonstrated the formation of functional cell spheroids above a PVA-coated surface may provide an in vitro system for investigating cell behaviors for tissue engineering of artificial salivary gland.
Authors:
Min-Huey Chen; Yi-Jane Chen; Chih-Chen Liao; Yen-Hui Chan; Chia-Yung Lin; Rung-Shu Chen; Tai-Hong Young
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  90     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1066-72     Citation Subset:  IM    
Copyright Information:
Copyright 2008 Wiley Periodicals, Inc.
Affiliation:
School of Dentistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Culture Techniques / methods
Coated Materials, Biocompatible / chemistry*
Parotid Gland / cytology*
Polyvinyl Alcohol*
Rats
Salivary Glands / cytology
Spheroids, Cellular / cytology*
Tissue Engineering / methods
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 9002-89-5/Polyvinyl Alcohol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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