Document Detail

Formation of reactive oxygen species in rat epithelial cells upon stimulation with fly ash.
MedLine Citation:
PMID:  12682424     Owner:  NLM     Status:  MEDLINE    
Fly ash was used as a model for ambient particulate matter which is under suspicion to cause adverse pulmonary health effects. The fly ash was pre-sized and contained only particles < 20 microm including an ultrafine fraction (< 100 nm) that contributed 31% to the particle number. In our study, we investigated the influence of fly ash on the promotion of early inflammatory reactions like the formation of reactive oxygen species (ROS) in rat lung epithelial cells (RLE-6TN). Furthermore, we determined the formation of nitric oxide (NO). The cells show a clear dose-response relationship concerning the formation of ROS with regard to the mass of particles applied. Lipopolysaccharide (LPS) added as a co-stimulus did not increase the formation of ROS induced by fly ash. Furthermore, in LPS (0.1 microg/ml) and tumour necrosis factor-alpha (TNF-alpha; 1 ng/ml) pre-treated cells no increase in reactive oxygen species comparable to fly ash alone is observable. In presence of the metal chelator, desferrioxamine (DFO), ROS formation can be significantly reduced. Neither fly ash nor LPS induced a significant NO release in RLE-6TN cells.
K Voelkel; H F Krug; S Diabaté
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biosciences     Volume:  28     ISSN:  0250-5991     ISO Abbreviation:  J. Biosci.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-04-08     Completed Date:  2004-09-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  51-5     Citation Subset:  IM    
Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, D-76021 Karlsruhe, Germany,
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MeSH Terms
Air Pollutants / pharmacology*
Carbon / pharmacology*
Cell Line
Cell Transformation, Viral
Chelating Agents / pharmacology
Culture Media / chemistry
Deferoxamine / pharmacology
Dose-Response Relationship, Drug
Epithelial Cells / drug effects,  metabolism*
Lipopolysaccharides / pharmacology
Lung / cytology
Nitric Oxide / biosynthesis
Nitrites / analysis
Particle Size
Particulate Matter
Reactive Oxygen Species / metabolism*
Tetradecanoylphorbol Acetate / pharmacology
Tumor Necrosis Factor-alpha / pharmacology
Reg. No./Substance:
0/Air Pollutants; 0/Chelating Agents; 0/Culture Media; 0/Lipopolysaccharides; 0/Nitrites; 0/Particulate Matter; 0/Reactive Oxygen Species; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 16561-29-8/Tetradecanoylphorbol Acetate; 68131-74-8/fly ash; 70-51-9/Deferoxamine; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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