Document Detail


Formation of the rabbit zona pellucida and its relationship to ovarian follicular development.
MedLine Citation:
PMID:  6386569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The zona pellucida is the unique extracellular glycoprotein matrix which is assembled during growth of the mammalian oocyte. The present studies were carried out to examine the formation of this structure in relation to the differentiation of ovarian cell types during follicular development. Specific antibodies were developed against total rabbit ZP proteins as well as against ZP proteins electrophoretically purified by high-resolution two-dimensional polyacrylamide electrophoresis gels (2D-PAGE). Antibodies were characterized by (a) immunoelectrophoresis, (b) a Staphylococcus aureus protein A binding assay, and (c) immunoblotting following 2D-PAGE separation of ZP proteins. Immunoperoxidase localization with these antibodies was used to determine the stage of ovarian follicular development at which ZP antigens first appear as well as to evaluate the cellular and extracellular distribution of these proteins throughout folliculogenesis. The ZP proteins were first observed in the cytoplasm and at the periphery of the oocytes surrounded by a thin squamous follicular cell layer. No staining was observed in the cytoplasm of follicle cells during early folliculogenesis. As the ZP matrix was assembled extracellularly, the intensity of staining of the outer and inner regions could be distinguished. This differentiation of the matrix coincided with the differentiation of the follicular cells into a multilayer cell complex. At this stage, specific ZP proteins are localized within the cytoplasm of the inner layers of these follicular cells. The staining is then diminished in cells of preantral follicles. These studies demonstrate that the formation of the ZP is an excellent model system to study the early stages of follicular development and cell differentiation.
Authors:
D J Wolgemuth; J Celenza; D S Bundman; B S Dunbar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  106     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-12-14     Completed Date:  1984-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-14     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / analysis
Electrophoresis, Polyacrylamide Gel
Female
Glycoproteins / analysis
Immune Sera
Immunosorbent Techniques
Ovarian Follicle / growth & development*
Ovum / analysis*
Rabbits
Tissue Distribution
Zona Pellucida / analysis*,  immunology
Chemical
Reg. No./Substance:
0/Antigens; 0/Glycoproteins; 0/Immune Sera

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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