Document Detail

Formation of phosphatidic acid in stressed mitochondria.
MedLine Citation:
PMID:  18835242     Owner:  NLM     Status:  MEDLINE    
Mitochondria are an important intracellular source of ROS as well as a sensitive target for oxidative damage under certain pathological conditions such as iron or copper overload. Mitochondrial membranes are rich in the tetraacyl phospholipid cardiolipin. Its integrity is important for efficient oxidative phosphorylation. Mouse liver mitochondria were subjected to oxidative stress by the Cu(2+)(Fe(2+))/H(2)O(2)/ascorbate system. Phosphatidic acid was detected in oxidized mitochondria, but not in unperturbed mitochondria. The Cu(2+)/H(2)O(2)/and (or not) ascorbate system caused the formation of phosphatidic acid and phosphatidylhydroxyacetone in cardiolipin liposomes. These products proceed via an HO*-radical induced fragmentation taking place in the polar moiety of cardiolipin. Mass spectrometry analysis of phosphatidic acid newly formed in mitochondria revealed that it has been derived from fragmentation of cardiolipin. Thus, free-radical fragmentation of cardiolipin in its polar part with the formation of phosphatidic acid is a likely mechanism that damages mitochondria under conditions of oxidative stress.
Irina L Yurkova; Franziska Stuckert; Mikhail A Kisel; Oleg I Shadyro; Juergen Arnhold; Dominik Huster
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-22
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  480     ISSN:  1096-0384     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-13     Completed Date:  2008-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17-26     Citation Subset:  IM    
Research Institute for Physical Chemical Problems of Belarusian State University, Minsk, Belarus.
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MeSH Terms
Biophysical Phenomena
Cardiolipins / metabolism
Chromatography, High Pressure Liquid
Iron / toxicity
Metals / toxicity
Mice, Inbred C57BL
Mitochondria, Liver / drug effects,  metabolism*
Oxidative Stress
Phosphatidic Acids / biosynthesis*
Reactive Oxygen Species / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Reg. No./Substance:
0/Cardiolipins; 0/Liposomes; 0/Metals; 0/Phosphatidic Acids; 0/Reactive Oxygen Species; 7439-89-6/Iron

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