| Formation of phosphatidic acid in stressed mitochondria. | |
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MedLine Citation:
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PMID: 18835242 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondria are an important intracellular source of ROS as well as a sensitive target for oxidative damage under certain pathological conditions such as iron or copper overload. Mitochondrial membranes are rich in the tetraacyl phospholipid cardiolipin. Its integrity is important for efficient oxidative phosphorylation. Mouse liver mitochondria were subjected to oxidative stress by the Cu(2+)(Fe(2+))/H(2)O(2)/ascorbate system. Phosphatidic acid was detected in oxidized mitochondria, but not in unperturbed mitochondria. The Cu(2+)/H(2)O(2)/and (or not) ascorbate system caused the formation of phosphatidic acid and phosphatidylhydroxyacetone in cardiolipin liposomes. These products proceed via an HO*-radical induced fragmentation taking place in the polar moiety of cardiolipin. Mass spectrometry analysis of phosphatidic acid newly formed in mitochondria revealed that it has been derived from fragmentation of cardiolipin. Thus, free-radical fragmentation of cardiolipin in its polar part with the formation of phosphatidic acid is a likely mechanism that damages mitochondria under conditions of oxidative stress. |
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Authors:
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Irina L Yurkova; Franziska Stuckert; Mikhail A Kisel; Oleg I Shadyro; Juergen Arnhold; Dominik Huster |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-22 |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 480 ISSN: 1096-0384 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-13 Completed Date: 2008-12-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 17-26 Citation Subset: IM |
Affiliation:
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Research Institute for Physical Chemical Problems of Belarusian State University, Minsk, Belarus. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biophysical Phenomena Cardiolipins / metabolism Chromatography, High Pressure Liquid Female Iron / toxicity Liposomes Metals / toxicity Mice Mice, Inbred C57BL Mitochondria, Liver / drug effects, metabolism* Oxidative Stress Phosphatidic Acids / biosynthesis* Reactive Oxygen Species / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Chemical | |
Reg. No./Substance:
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0/Cardiolipins; 0/Liposomes; 0/Metals; 0/Phosphatidic Acids; 0/Reactive Oxygen Species; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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