| Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite. | |
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MedLine Citation:
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PMID: 22376028 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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CEB25 is a human minisatellite locus, composed of slightly polymorphic 52-nt tandem repeats. Genetically, most if not all individuals of the human population are heterozygous, carrying alleles ranging from 0.5 kb up to 20 kb, maintained by mendelian inheritance but also subject to germline instability. To provide insights of the CEB25 biological role, we interrogated its structural features. We report the NMR structure of the G-quadruplex formed by the conserved 26-nt G-rich fragment of the CEB25 motif. In K+ solution, this sequence forms a propeller-type parallel-stranded G-quadruplex involving a 9-nt central double-chain-reversal loop. This long loop is anchored to the 5' end of the sequence by an A•T Watson-Crick and a potential G•A non-canonical base pairs. These base pairs contribute to the stability of the overall G-quadruplex structure, as measured by an increase of about 17 kcal/mol in enthalpy or 6°C in melting temperature. Further, we demonstrate that such a monomorphic structure is formed within longer sequence contexts folding into a pearl-necklace structure. |
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Authors:
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Samir Amrane; Michael Adrian; Brahim Heddi; Alexandre Serero; Alain Nicolas; Jean-Louis Mergny; Anh Tuan Phan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-29 |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: - ISSN: 1520-5126 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-3-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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