| Formation of oxygen radicals in solutions of 7,8-dihydroneopterin. | |
| | |
MedLine Citation:
|
PMID: 10527875 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Neopterin and 7,8-dihydroneopterin, two compounds which are secreted by activated macrophages, have been shown to interfere with radicals generated by cellular and certain chemical systems. Reduced pterins were reported to scavenge whereas aromatic pterins promoted or reduced radical mediated reactions or had no effect. However, recently it was found that high concentrations of 7, 8-dihydroneopterin enhanced luminol dependent chemiluminescence and T-cell apoptosis, suggesting an enhancement of free radical formation. In this study hydroxylation of salicylic acid was used for detection of hydroxyl radicals. It is shown that in solutions of 7,8-dihydroneopterin hydroxyl radicals were formed in the absence of any radical source. The presence of EDTA chelated iron enhanced hydroxyl radical formation. Whereas the addition of iron accelerated the hydroxylation reaction, 7,8-dihydroneopterin was responsible for the amount of hydroxylation products. In the presence of superoxide dismutase or catalase, as well as by helium purging, hydroxylation was inhibited. Our data suggest that in solutions of 7, 8-dihydroneopterin superoxide radicals are generated which are converted to hydroxyl radicals by Fenton or Haber-Weiss type reactions. While superoxide might be generated during autoxidation of ferrous iron, dihydroneopterin seems to be involved in regeneration of ferrous iron from the ferric form. |
| | |
Authors:
|
K Oettl; B Wirleitner; G Baier-Bitterlich; T Grammer; D Fuchs; G Reibnegger |
Related Documents
:
|
19785155 - Oligomerization and cyclization reactions of acetylene. 11697125 - A novel method of measuring hydroxyl radical-scavenging activity of antioxidants using ... 1575505 - Oxidation of methyl linoleate in aqueous dispersions induced by copper and iron. 12785055 - Singlet oxygen-mediated hydroxyl radical production in the presence of phenols: whether... 23614755 - A new directing mode for singlet oxygen ene reactions: the vinylogous gem effect enable... 17152355 - Role of extracellular and intracellular nitric oxide in the regulation of macrophage re... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 264 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1999 Oct |
Date Detail:
|
Created Date: 1999-11-12 Completed Date: 1999-11-12 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 262-7 Citation Subset: IM |
Copyright Information:
|
Copyright 1999 Academic Press. |
Affiliation:
|
Institute for Medical Chemistry and Pregl Laboratory, Karl-Franzens-University Graz, Harrachgasse 21/II, Graz, A-8010, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Catalase
/
metabolism Chromatography, High Pressure Liquid Edetic Acid / chemistry Hydroxyl Radical / metabolism* Hydroxylation Iron / chemistry Neopterin / metabolism Oxygen / metabolism* Pteridines / metabolism* Superoxide Dismutase / metabolism Superoxides / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Pteridines; 11062-77-4/Superoxides; 1218-98-0/7,8-dihydroneopterin; 3352-57-6/Hydroxyl Radical; 60-00-4/Edetic Acid; 670-65-5/Neopterin; 7439-89-6/Iron; 7782-44-7/Oxygen; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Enhancement of dendrimer-mediated transfection using synthetic lung surfactant exosurf neonatal in v...
Next Document: Interaction of measles virus (Hallé strain) with CD46: evidence that a common binding site on CD46 ...