Document Detail


Formation of ovarian follicular fluid may be due to the osmotic potential of large glycosaminoglycans and proteoglycans.
MedLine Citation:
PMID:  16816338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During mammalian follicle development, a fluid-filled antrum develops in the avascular centre of the follicle. We investigated the hypothesis that follicular fluid contains osmotically-active molecules, sufficiently large so as to not freely escape the follicular fluid. Such molecules could generate an osmotic differential and thus recruit fluid from the surrounding vascularised stroma into the antrum. Follicular fluid was collected from bovine follicles classified histologically as healthy (n = 4 pools) or atretic (n = 4 pools). Dialysis of the follicular fluid at 300 kDa or 500 kDa resulted in a reduction in colloid osmotic pressure of 35% and 60%, respectively, in fluid from healthy follicles and 29% and 80% from atretic follicles. Digestion of follicular fluid with Streptomyces hyaluronidase, chondroitinase ABC or DNase 1 followed by dialysis resulted in reductions in osmotic pressure of 43%, 53% and 43% respectively for fluids from healthy follicles and 34%, 20% and 31% for atretic follicles. Digestion with collagenase I, proteinase K, heparanase 1 or keratanase had no significant effect on the osmotic pressure of follicular fluid of healthy follicles. Ion exchange and size exclusion, Western blotting and ELISA identified the proteoglycans versican and inter-alpha trypsin inhibitor and the glycosaminoglycan hyaluronan in follicular fluid. We conclude that these molecules or aggregates of them are of sufficient size to contribute to the osmotic potential of follicular fluid and thus recruit fluid into the follicular antrum. DNA may also contribute but it is probably not a component that is regulated for this role.
Authors:
Hannah G Clarke; Sarah A Hope; Sharon Byers; Raymond J Rodgers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  132     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-03     Completed Date:  2006-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  119-31     Citation Subset:  IM    
Affiliation:
Research Centre for Reproductive Health, Department of Obstetrics and Gynaecology, The University of Adelaide, Adelaide, SA 5005, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western / methods
Cattle
Cell Membrane / metabolism
Chromatography, Ion Exchange
DNA / analysis
Dialysis
Digestion
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay / methods
Enzymes / metabolism
Female
Follicular Fluid / metabolism*
Glycosaminoglycans / analysis*
Osmosis
Ovarian Follicle / metabolism*
Proteoglycans / analysis*
Chemical
Reg. No./Substance:
0/Enzymes; 0/Glycosaminoglycans; 0/Proteoglycans; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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