Document Detail


Formation of novel central and peripheral connections between molluscan central neurons in organ cultured ganglia.
MedLine Citation:
PMID:  7077319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An in vitro organ culture system for buccal ganglia of the adult snail, Helisoma, is described. The system supports: (1) maintenance of characteristic electrophysiological parameters of identified neurons over seven days of culture; (2) choline metabolism including uptake and synthesis over the same duration; (3) sprouting and growth of neurons in response to axotomy; (4) the formation of novel central electrotonic connections between identified neurons as a result of sprouting and growth. These observations on neuronal growth and the formation of connections are similar to those made with in vivo culture. The use of in vitro culture allows precise manipulations not previously possible. When buccal ganglia are cultured in vitro with the cut distal ends of peripheral nerve trunks held closely apposed, axons of neurons 5R and 5L in the nerve trunks are capable of forming electrotonic connections similar to central connections. The capability of these neurons to form electrotonic connections via their peripheral axons implies that special structures (i.e., central neurites) are not required for the formation of connections; and neither are special environments (i.e., the central neuropile) required for these connections.
Authors:
R D Hadley; R G Wong; S B Kater; D L Barker; A G Bulloch
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurobiology     Volume:  13     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-07-19     Completed Date:  1982-07-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  217-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Central Nervous System / anatomy & histology,  cytology*
Cheek / innervation
Ganglia / cytology,  physiology*
Mollusca / physiology*
Neural Pathways / physiology
Neurons / physiology*
Organ Culture Techniques
Grant Support
ID/Acronym/Agency:
MH 15172/MH/NIMH NIH HHS; NS 15350/NS/NINDS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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