| Formation of multicellular spheroids composed of rat hepatocytes. | |
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MedLine Citation:
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PMID: 1439174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel method for the preparation of spheroids containing two types of cells (hetero-spheroid) has been successfully developed by using a collagen-conjugated thermo-responsive polymer, poly-N-isopropyl acrylamide, as a cell substratum. The hetero-spheroid was prepared by detaching the confluent monolayer composed of parenchymal and non-parenchymal rat liver cells at a temperature below lower critical solution temperature and culturing it on the non-adhesive substratum. The hetero-spheroid had activity in albumin secretion and PNPA hydrolase activity over a period of 60 days in dishes. These findings suggest that this spheroid formation system is a useful model of an alternative to animal tests of hepatotoxicity. |
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Authors:
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K Ueno; A Miyashita; E Endoh; T Takezawa; M Yamazaki; Y Mori; T Satoh |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Research communications in chemical pathology and pharmacology Volume: 77 ISSN: 0034-5164 ISO Abbreviation: Res. Commun. Chem. Pathol. Pharmacol. Publication Date: 1992 Jul |
Date Detail:
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Created Date: 1992-12-07 Completed Date: 1992-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0244734 Medline TA: Res Commun Chem Pathol Pharmacol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 107-20 Citation Subset: IM |
Affiliation:
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Laboratory of Biochemical Pharmacology & Bio-Toxicology, Faculty of Pharmaceutical Sciences, Chiba University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins Albumins / secretion Animals Carboxylic Ester Hydrolases / metabolism Cell Adhesion Cells, Cultured Culture Media Cytological Techniques Liver / cytology*, enzymology, secretion Rats |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Albumins; 0/Culture Media; 25189-55-3/poly-N-isopropylacrylamide; EC 3.1.1.-/4-nitrophenyl acetate hydrolase; EC 3.1.1.-/Carboxylic Ester Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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