Document Detail


Formation of multicellular aggregates under different conditions of microenvironment.
MedLine Citation:
PMID:  20201409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multicellular aggregates (spheroids) represent an intermittent level between monolayer growing cells and tissue culture. Spheroids are rather objective model of the three-dimensional growth and organization, the cell-to-cell interactions and influence of microenvironmental conditions on tumour microaggregates. In our work formation and growth of spheroids depends on concentration of CMC and FCS. Conditions of microenvironment influence on intensiveness of proliferation as well as on cells adhesiveness and formation of microaggregates.
Authors:
L Garmanchouk; O Perepelytsina; M Sydorenko; L I Ostapchenko
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  T?Sitologii?a i genetika     Volume:  44     ISSN:  0564-3783     ISO Abbreviation:  Tsitol. Genet.     Publication Date:    2010 Jan-Feb
Date Detail:
Created Date:  2010-03-05     Completed Date:  2010-05-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0101671     Medline TA:  Tsitol Genet     Country:  Ukraine    
Other Details:
Languages:  eng     Pagination:  25-9     Citation Subset:  IM    
Affiliation:
Department of Biotechnical Problems of Diagnostics, Institute problems of Cryobiology and Criomedicine NAN, Ukraine, Kyiv.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carboxymethylcellulose / chemistry
Cattle
Cell Adhesion / physiology
Cell Culture Techniques / methods
Cell Line, Tumor
Cell Proliferation
Culture Media / chemistry
Fetal Blood / chemistry
Humans
Models, Biological*
Serum / chemistry
Spheroids, Cellular / cytology*,  pathology
Chemical
Reg. No./Substance:
0/Culture Media; 9004-32-4/Carboxymethylcellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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