Document Detail

Formation of molecular species of mitochondrial cardiolipin. 1. A novel transacylation mechanism to shuttle fatty acids between sn-1 and sn-2 positions of multiple phospholipid species.
MedLine Citation:
PMID:  19416660     Owner:  NLM     Status:  MEDLINE    
Mitochondrial cardiolipin undergoes extensive remodeling of its acyl groups to generate uniformly substituted species, such as tetralinoleoyl-cardiolipin, but the mechanism of this remodeling has not been elucidated, except for the fact that it requires tafazzin. Here we show that purified recombinant Drosophila tafazzin exchanges acyl groups between cardiolipin and phosphatidylcholine by a combination of forward and reverse transacylations. The acyl exchange is possible in the absence of phospholipase A(2) because it requires only trace amounts of lysophospholipids. We show that purified tafazzin reacts with various phospholipid classes and with various acyl groups both in sn-1 and sn-2 position. Expression studies in Sf9 insect cells suggest that the effect of tafazzin on cardiolipin species is dependent on the cellular environment and not on enzymatic substrate specificity. Our data demonstrate that tafazzin catalyzes general acyl exchange between phospholipids, which raises the question whether pattern formation in cardiolipin is the result of the equilibrium distribution of acyl groups between multiple phospholipid species.
Ashim Malhotra; Yang Xu; Mindong Ren; Michael Schlame
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-01-21
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1791     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-08     Completed Date:  2009-06-30     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  314-20     Citation Subset:  IM    
Department of Anesthesiology, New York University Langone Medical Center, New York, NY 10016, USA.
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MeSH Terms
1-Acylglycerophosphocholine O-Acyltransferase / metabolism
Cardiolipins / metabolism*
Drosophila Proteins / metabolism
Drosophila melanogaster / metabolism*
Fatty Acids / metabolism*
Mitochondria, Liver / metabolism*
Phosphatidylcholines / metabolism*
Phospholipases A2 / antagonists & inhibitors,  metabolism
Grant Support
R01 HL078788-01/HL/NHLBI NIH HHS; R01 HL078788-01A1/HL/NHLBI NIH HHS; R01 HL078788-02/HL/NHLBI NIH HHS; R01 HL078788-03/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Cardiolipins; 0/Drosophila Proteins; 0/Fatty Acids; 0/Phosphatidylcholines; EC O-Acyltransferase; EC protein, Drosophila; EC A2

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