Document Detail


Formation of lipoidal steroids in follicular fluid.
MedLine Citation:
PMID:  2770299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of high levels of lipoidal pregnenolone in follicular fluid has recently been established although no evidence has been presented concerning its possible origin. The following investigation focuses on the enzymatic conversion of non-conjugated steroids into their lipoidal derivatives in preovulatory follicular fluid obtained from women undergoing in vitro fertilization. Our observations indicated that pregnenolone, an important precursor steroid, was acylated at a similar rate as cholesterol in follicular fluid. Similar studies were subsequently conducted with serum obtained from a pool of normal women and women undergoing follicular stimulation which showed little difference to the results obtained in follicular fluid. Further studies using dehydroepiandrosterone, androst-5-ene-3 beta,17 beta-diol, estradiol and dihydrotestosterone were were also performed to monitor their respective lipoidal conversion percentages in follicular fluid which revealed a marked difference of conversion rates between steroids. The indirect identification of the lipoidal pregnenolone derivatives formed in follicular fluid was also conducted by incubating radiolabelled pregnenolone in follicular fluid. The fatty acid components of the resulting lipoidal pregnenolone derivatives showed a marked resemblance to those of cholesteryl esters formed in plasma by the enzymatic activity of lecithin:cholesterol acyltransferase. The pregnenolone derivatives were comprised predominantly of unsaturated fatty acids such as linoleate, palmitoleate, oleate, linolenate and arachidonate while saturated fatty acids, namely palmitate, constituted 20% of the total lipoidal pregnenolone.
Authors:
R Roy; A Bélanger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of steroid biochemistry     Volume:  33     ISSN:  0022-4731     ISO Abbreviation:  J. Steroid Biochem.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-29     Completed Date:  1989-09-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0260125     Medline TA:  J Steroid Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  257-62     Citation Subset:  IM    
Affiliation:
MRC Group in Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Ste-Foy, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Chromatography, Liquid
Female
Humans
Lipids / biosynthesis*,  blood
Ovarian Follicle / metabolism*
Pregnenolone / biosynthesis*,  blood
Chemical
Reg. No./Substance:
0/Lipids; 145-13-1/Pregnenolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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