Document Detail

Formation of leukotrienes E3, E4 and E5 in rat basophilic leukemia cells.
MedLine Citation:
PMID:  6273168     Owner:  NLM     Status:  MEDLINE    
Rat basophilic leukemia (RBL-1) cells incubated with ionophore A23187 and 5,8,11-eicosatrienoic acid produced three slow-reacting substances identified as leukotrienes C3, D3 and E3 by spectroscopic, chromatographic and enzymatic methods. 5,8,11,14,17-Eicosapentaenoic acid was similarly converted by RBL-1 cells to leukotrienes C5, D5. and E5. Leukotrienes C4, D4 and E4 were also formed in these experiments from endogenous arachidonic acid. Time-course studies, incubations with 3H-labeled leukotriene C3 and effects of acivicin [L-(alpha S, 5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid; a gamma-glutamyl transpeptidase inhibitor] indicated that leukotrienes C and D are intermediates in the formation of leukotrienes E. L-Cysteine enhanced the conversion of leukotriene C3 to leukotriene D3 and inhibited further degradation of leukotriene D3 to leukotriene E3.
L Orning; K Bernström; S Hammarström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  120     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1982-02-12     Completed Date:  1982-02-12     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  41-5     Citation Subset:  IM    
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MeSH Terms
8,11,14-Eicosatrienoic Acid / pharmacology
Arachidonic Acid
Arachidonic Acids / blood*,  metabolism
Basophils / metabolism*
Calcimycin / pharmacology
Chromatography, High Pressure Liquid
Cysteine / pharmacology
Glycine / analogs & derivatives,  pharmacology
Isoxazoles / pharmacology
Leukemia, Experimental / metabolism*
Leukotriene E4
SRS-A / blood*
Reg. No./Substance:
0/Arachidonic Acids; 0/Isoxazoles; 0/SRS-A; 2751-14-6/mead acid; 506-32-1/Arachidonic Acid; 52-90-4/Cysteine; 52583-41-2/acivicin; 52665-69-7/Calcimycin; 56-40-6/Glycine; 7324-41-6/8,11,14-Eicosatrienoic Acid; 75715-89-8/Leukotriene E4

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