Document Detail


Formation of composite polyacrylamide and silicone substrates for independent control of stiffness and strain.
MedLine Citation:
PMID:  23287818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells that line major tissues in the body such as blood vessels, lungs and gastrointestinal tract experience deformation from mechanical strain with our heartbeat, breathing, and other daily activities. Tissues also remodel in both development and disease, changing their mechanical properties. Taken together, cells can experience vastly different mechanical cues resulting from the combination of these interdependent stimuli. To date, most studies of cellular mechanotransduction have been limited to assays in which variations in substrate stiffness and strain were not combined. Here, we address this technological gap by implementing a method that can simultaneously tune both substrate stiffness and mechanical strain. Substrate stiffness is controlled with different monomer and crosslinker ratios during polyacrylamide gel polymerization, and strain is transferred from the underlying silicone platform when stretched. We demonstrate this platform with polyacrylamide gels with elastic moduli at 6 kPa and 20 kPa in combination with two different silicone formulations. The gels remain attached with up to 50% applied strains. To validate strain transfer through the gels into cells, we employ particle-tracking methods and observe strain transmission via cell morphological changes.
Authors:
Chelsey S Simmons; Alexandre J S Ribeiro; Beth L Pruitt
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-24     Completed Date:  2013-10-30     Revised Date:  2014-02-23    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  646-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / chemical synthesis,  chemistry*
Cell Adhesion
Cell Culture Techniques / methods*
Cells, Cultured
Endothelial Cells / cytology*
Humans
Muscle, Smooth, Vascular / cytology*
Silicones / chemical synthesis,  chemistry*
Grant Support
ID/Acronym/Agency:
EB006745/EB/NIBIB NIH HHS; HL089027/HL/NHLBI NIH HHS; R01 EB006745/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Silicones; 9003-05-8/polyacrylamide
Comments/Corrections

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