Document Detail


Formation and characterization of iron-binding phosphorylated human-like collagen as a potential iron supplement.
MedLine Citation:
PMID:  23910354     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Iron incorporated into food can induce precipitation and unwanted interaction with other components in food. Iron-binding proteins represent a possibility to avoid these problems and other side effects, as the iron is protected. However, there are several technical problems associated with protein-iron complex formation. In this paper, the iron-binding phosphorylated human-like collagen (Fe-G6P-HLC) was prepared under physiological conditions through phosphorylated modification. One molecule of Fe-G6P-HLC possesses about 24 atoms of Fe. Spectroscopy analysis, differential scanning calorimetry (DSC) and equilibrium dialysis techniques were employed to investigate the characteristics of the Fe-G6P-HLC. The binding sites (nb) and apparent association constant (Kapp) between iron and phosphorylated HLC were measured at nb=23.7 and log Kapp=4.57, respectively. The amount of iron (Fe(2+) sulfate) binding to phosphorylated HLC was found to be a function of pH and phosphate content. In addition, the solubility and thermal stability of HLC were not significantly affected. The results should facilitate the utilization of HLC as a bioactive iron supplement in the food and medical industry and provide an important theoretical evidence for the application of HLC chelates.
Authors:
Jianjun Deng; Fei Chen; Daidi Fan; Chenhui Zhu; Xiaoxuan Ma; Wenjiao Xue
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Publication Detail:
Type:  Journal Article     Date:  2013-06-28
Journal Detail:
Title:  Materials science & engineering. C, Materials for biological applications     Volume:  33     ISSN:  1873-0191     ISO Abbreviation:  Mater Sci Eng C Mater Biol Appl     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-08-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101484109     Medline TA:  Mater Sci Eng C Mater Biol Appl     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  4361-8     Citation Subset:  IM    
Copyright Information:
© 2013.
Affiliation:
Shaanxi Key Laboratory of Degradable Biomedical Materials, College of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, College of Chemical Engineering, Xi'an 710069, China.
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