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Formation of Charcot-Leyden crystals by human basophils.
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MedLine Citation:
PMID:  7042896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Charcot-Leyden crystals (CLC) are currently believed to be unique to the eosinophil and a hallmark of active eosinophilic inflammation or proliferation. The distinctiveness of the CLC to the eosinophil was questioned in 1965 by Archer and Blackwood (9), but their demonstration of CLC formation in basophils was ignored and later dismissed (1) as being the result of eosinophil contamination of basophil-enriched cell suspensions. We reexamined this question and showed that basophils obtained from the peripheral blood of normal individuals form CLC and that basophils contain a protein that is immunochemically indistinguishable from eosinophil CLC protein. These conclusions are based upon the findings that (a) crystal formation in basophils was demonstrated by specific histochemical staining of crystal-containing cells in highly enriched basophil suspensions prepared by fluorescence-activated cell sorter (FACS) purification of surface IgE-positive cells, (b) that enrichment for surface IgE-positive cells (primarily basophils) by the FACS also enriched for cells staining positively by immunofluorescence for eosinophil CLC protein, and (c) that CLC protein was measured by radioimmunoassay in cell extracts prepared from purified basophil suspensions containing 97-99% basophils and absolutely no contaminating eosinophils. These basophil extracts contained a protein immunochemically indistinguishable from eosinophil CLC protein. Based upon these findings, the CLC or the protein comprising the crystal (lysophospholipase) can no longer be considered as distinctive to the eosinophil. We must now consider the possibility that the presence of CLC in tissues, sputum, or stool may also represent basophil involvement in disease processes.
Authors:
S J Ackerman; G J Weil; G J Gleich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  155     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1982 Jun 
Date Detail:
Created Date:  1982-07-19     Completed Date:  1982-07-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1597-609     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Basophils / analysis*,  metabolism
Cell Separation
Eosinophils / analysis
Flow Cytometry
Fluorescent Antibody Technique
Freezing
Glycoproteins / analysis,  biosynthesis*
Humans
Lysophospholipase
Rabbits
Grant Support
ID/Acronym/Agency:
AI 07047/AI/NIAID NIH HHS; AI 15231/AI/NIAID NIH HHS; AI 9728/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/lysolecithin acylhydrolase; EC 3.1.1.5/Lysophospholipase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 6 Year: 1982
Volume: 155 Issue: 6
First Page: 1597 Last Page: 1609
ID: 2186692
Publisher Id: 82191417
PubMed Id: 7042896

Formation of Charcot-Leyden crystals by human basophils


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