| Formate buffer as preservative for urinary free catecholamine measurement. | |
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MedLine Citation:
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PMID: 14713383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Strong mineral acid is the most commonly used preservative for measuring urinary free catecholamines. Leakage of corrosive acid is a safety hazard. The use of formate buffer as a safer alternative was studied. METHODS: Twenty-two urine specimens from post-operative patients were collected and preserved in 0.5 M hydrochloric acid or 0.75 M formate buffer. The specimens were stored at 4 degrees C or -70 degrees C, respectively. The free catecholamine content was measured at regular intervals by high-performance liquid chromatography for 6 months. RESULTS: The preservation capability of formate buffer was equivalent to that of hydrochloric acid. Deep-freeze storage offered additional protection independent of the preservative used. CONCLUSION: The results of this study demonstrate that formate buffer, compared to the most popularly used strong mineral acid solution, is an equally effective preservation for urinary free catecholamines. The less acidic nature of formate should invite fewer unpleasant incidents and safety hazards. |
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Authors:
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Y P Iu; C S Ho; T W L Mak |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of clinical biochemistry Volume: 41 ISSN: 0004-5632 ISO Abbreviation: Ann. Clin. Biochem. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-01-09 Completed Date: 2004-09-10 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0324055 Medline TA: Ann Clin Biochem Country: England |
Other Details:
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Languages: eng Pagination: 39-42 Citation Subset: IM |
Affiliation:
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Department of Clinical Pathology, North District Hospital, Hong Kong Special Administrative Region, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Buffers Catecholamines / urine* Dopamine / urine Epinephrine / urine Formic Acids / chemistry* Humans Norepinephrine / urine Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Catecholamines; 0/Formic Acids; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 64-18-6/formic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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