Document Detail

Formate buffer as preservative for urinary free catecholamine measurement.
MedLine Citation:
PMID:  14713383     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Strong mineral acid is the most commonly used preservative for measuring urinary free catecholamines. Leakage of corrosive acid is a safety hazard. The use of formate buffer as a safer alternative was studied. METHODS: Twenty-two urine specimens from post-operative patients were collected and preserved in 0.5 M hydrochloric acid or 0.75 M formate buffer. The specimens were stored at 4 degrees C or -70 degrees C, respectively. The free catecholamine content was measured at regular intervals by high-performance liquid chromatography for 6 months. RESULTS: The preservation capability of formate buffer was equivalent to that of hydrochloric acid. Deep-freeze storage offered additional protection independent of the preservative used. CONCLUSION: The results of this study demonstrate that formate buffer, compared to the most popularly used strong mineral acid solution, is an equally effective preservation for urinary free catecholamines. The less acidic nature of formate should invite fewer unpleasant incidents and safety hazards.
Y P Iu; C S Ho; T W L Mak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of clinical biochemistry     Volume:  41     ISSN:  0004-5632     ISO Abbreviation:  Ann. Clin. Biochem.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-09     Completed Date:  2004-09-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0324055     Medline TA:  Ann Clin Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  39-42     Citation Subset:  IM    
Department of Clinical Pathology, North District Hospital, Hong Kong Special Administrative Region, China.
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MeSH Terms
Catecholamines / urine*
Dopamine / urine
Epinephrine / urine
Formic Acids / chemistry*
Norepinephrine / urine
Time Factors
Reg. No./Substance:
0/Buffers; 0/Catecholamines; 0/Formic Acids; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 64-18-6/formic acid

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