| Formate as an auxiliary substrate for glucose-limited cultivation of Penicillium chrysogenum: impact on penicillin G production and biomass yield. | |
| | |
MedLine Citation:
|
PMID: 17545326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Production of beta-lactams by the filamentous fungus Penicillium chrysogenum requires a substantial input of ATP. During glucose-limited growth, this ATP is derived from glucose dissimilation, which reduces the product yield on glucose. The present study has investigated whether penicillin G yields on glucose can be enhanced by cofeeding of an auxiliary substrate that acts as an energy source but not as a carbon substrate. As a model system, a high-producing industrial strain of P. chrysogenum was grown in chemostat cultures on mixed substrates containing different molar ratios of formate and glucose. Up to a formate-to-glucose ratio of 4.5 mol.mol(-1), an increasing rate of formate oxidation via a cytosolic NAD(+)-dependent formate dehydrogenase increasingly replaced the dissimilatory flow of glucose. This resulted in increased biomass yields on glucose. Since at these formate-to-glucose ratios the specific penicillin G production rate remained constant, the volumetric productivity increased. Metabolic modeling studies indicated that formate transport in P. chrysogenum does not require an input of free energy. At formate-to-glucose ratios above 4.5 mol.mol(-1), the residual formate concentrations in the cultures increased, probably due to kinetic constraints in the formate-oxidizing system. The accumulation of formate coincided with a loss of the coupling between formate oxidation and the production of biomass and penicillin G. These results demonstrate that, in principle, mixed-substrate feeding can be used to increase the yield on a carbon source of assimilatory products such as beta-lactams. |
| | |
Authors:
|
Diana M Harris; Zita A van der Krogt; Walter M van Gulik; Johannes P van Dijken; Jack T Pronk |
Related Documents
:
|
16912926 - Over-expression of glucose dehydrogenase improves cell growth and riboflavin production... 7556586 - Effect of glucose on the respiratory burst of circulating neutrophils from asthmatics. 16797976 - Microbial hydrogen production with bacillus coagulans iit-bt s1 isolated from anaerobic... 7870136 - Aerobic and anaerobic glucose metabolism of phytomonas sp. isolated from euphorbia char... 2577686 - Lidocaine treatment of painful diabetic neuropathy and endogenous opioid peptides in pl... 17558326 - Evaluation of costs for cystoid macular edema among patients after cataract surgery. |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-01 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 73 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-07-30 Completed Date: 2007-10-10 Revised Date: 2010-09-16 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 5020-5 Citation Subset: IM |
Affiliation:
|
Delft University of Technology, Department of Biotechnology, Julianalaan 67, Delft, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aerobiosis Biomass* Biotechnology / methods* Culture Media Formates / metabolism* Glucose / metabolism* Penicillin G / metabolism* Penicillium chrysogenum / growth & development*, metabolism beta-Lactams / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Formates; 0/beta-Lactams; 50-99-7/Glucose; 61-33-6/Penicillin G |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: NCgl2620 encodes a class II polyphosphate kinase in Corynebacterium glutamicum.
Next Document: Control of Mycosphaerella graminicola on wheat seedlings by medical drugs known to modulate the acti...