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Formaldehyde metabolism and formaldehyde-induced stimulation of lactate production and glutathione export in cultured neurons.
MedLine Citation:
PMID:  23356791     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Formaldehyde is endogenously produced in the human body and brain levels of this compound are elevated in neurodegenerative conditions. Although the toxic potential of an excess of formaldehyde has been studied, little is known on the molecular mechanisms underlying its neurotoxicity as well as on the ability of neurons to metabolize formaldehyde. To address these topics we have used cerebellar granule neuron cultures as model system. These cultures express mRNAs of various enzymes that are involved in formaldehyde metabolism and were remarkably resistant towards acute formaldehyde toxicity. Cerebellar granule neurons metabolized formaldehyde with a rate of around 200 nmol/(h x mg) which was accompanied by significant increases in the cellular and extracellular concentrations of formate. In addition, formaldehyde application significantly increased glucose consumption, almost doubled the rate of lactate release from viable neurons and strongly accelerated the export of the antioxidant glutathione. The latter process was completely prevented by inhibition of the known glutathione exporter multidrug resistance protein 1. These data indicate that cerebellar granule neurons are capable of metabolizing formaldehyde and that the neuronal glycolysis and glutathione export are severely affected by the presence of formaldehyde. © 2013 International Society for Neurochemistry, J. Neurochem. (2013) 10.1111/jnc.12170.
Authors:
Ketki Tulpule; Michaela C Hohnholt; Ralf Dringen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-29
Journal Detail:
Title:  Journal of neurochemistry     Volume:  -     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 International Society for Neurochemistry, J. Neurochem. (2013) 10.1111/jnc.12170.
Affiliation:
Centre for Biomolecular Interactions Bremen, University of Bremen, PO. Box 330440, D-28334, Bremen, Germany; Centre for Environmental Research and Sustainable Technology, Leobener Strasse, D-28359, Bremen, Germany.
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