Document Detail

Formaldehyde-induced acentric chromosome fragments and chromosome stickiness in Chortophaga neuroblasts.
MedLine Citation:
PMID:  3709425     Owner:  NLM     Status:  MEDLINE    
Embryos of the grasshopper Chortophaga viridifasciata were exposed in vitro to formaldehyde (FA), as formalin, at concentrations ranging from 10(-8)M (0.0003 ppm) to 10(-3) M (30 ppm) at 38 degrees C. A low frequency of distinct acentric chromosome fragments (0.02-0.04/cell) was observed in the neuroblasts after 1 hr exposure to 7.5 X 10(-4) or 10(-3) M FA plus 3 hr recovery, but not at lower concentrations, even with 4 hr exposure. There was no obvious relation between distinct fragment frequency and concentration of FA. Neuroblasts with sticky chromosomes were observed at 10(-4), 7.5 X 10(-4), and 10(-3) M FA, the percent of cells with slight, moderate, or severe stickiness varying with FA concentrations. Fragments were associated with the sticky chromosomes. The frequency of these sticky fragments at the two higher concentrations (0.15-0.30/cell) was greater than the frequency of distinct fragments. It is concluded that the distinct acentric fragments induced by FA result from breakage at a single sticky point (slight stickiness) between separating sister chromatids. The chromosome effects observed probably result from the action of daughter products that are formed by the interaction of FA with culture medium components, especially the fetal calf serum.
M A Dowd; M E Gaulden; B L Proctor; G B Seibert
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental mutagenesis     Volume:  8     ISSN:  0192-2521     ISO Abbreviation:  Environ Mutagen     Publication Date:  1986  
Date Detail:
Created Date:  1986-07-03     Completed Date:  1986-07-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909737     Medline TA:  Environ Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  401-11     Citation Subset:  IM    
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MeSH Terms
Chromosome Aberrations*
Chromosomes / drug effects*
Embryo, Nonmammalian / drug effects,  ultrastructure
Formaldehyde / pharmacology*
Grasshoppers / drug effects*,  embryology
Neurons / drug effects*,  ultrastructure
Organ Culture Techniques
Grant Support
Reg. No./Substance:

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