Document Detail


Formaldehyde fixation and microwave irradiation.
MedLine Citation:
PMID:  2464564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formaldehyde is the most commonly used fixative in pathology laboratories. However, due to time pressures, this fixative is often not optimally exploited. The majority of biopsies are only partly fixed when histoprocessing is started, with adverse effects. This paper reports how formaldehyde fixation is improved, by using 1.5 min of microwave irradiation of tissue previously soaked for four hours in the fixation solution. It is argued that this beneficial effect of microwave irradiation can be attributed to the acceleration of the reaction of formaldehyde to the tissue. Formation of free formaldehyde, by the dehydration of methylene glycol present in the tissue when the irradiation starts, is also enhanced. Five different formaldehyde-containing fixatives were evaluated, using five different working protocols. Spleen was taken as a suitable tissue for these tests. The technique described leads to uniform microscopical results. It is a simple method and is suitable for use in routine laboratories.
Authors:
M E Boon; P O Gerrits; H E Moorlag; P Nieuwenhuis; L P Kok
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Histochemical journal     Volume:  20     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:    1988 Jun-Jul
Date Detail:
Created Date:  1989-03-17     Completed Date:  1989-03-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  313-22     Citation Subset:  IM    
Affiliation:
Leiden Cytology and Pathology Laboratory, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Formaldehyde*
Histological Techniques*
Humans
Indicators and Reagents
Microwaves*
Staining and Labeling
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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