| Forkhead transcription factors contribute to execution of the mitotic programme in mammals. | |
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MedLine Citation:
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PMID: 11607034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle progression is a process that is tightly controlled by internal and external signals. Environmental cues, such as those provided by growth factors, activate early signals that promote cell cycle entry. Cells that have progressed past the restriction point become independent of growth factors, and cell cycle progression is then controlled endogenously. The phosphatidylinositol 3OH kinase (PI(3)K)/protein kinase B (PKB) pathway must be activated in G1 to inactivate forkhead transcription factors (FKH-TFs) and allow cell cycle entry. Here we show that subsequent attenuation of the PI(3)K/PKB pathway is required to allow transcriptional activation of FKH-TF in G2. FKH-TF activity in G2 controls mammalian cell cycle termination, as interference with FKH transcriptional activation by disrupting PI(3)K/PKB downregulation, or by expressing a transcriptionally inactive FKH mutant, induces cell accumulation in G2/M, defective cytokinesis, and delayed transition from M to G1 of the cell cycle. We demonstrate that FKH-TFs regulate expression of mitotic genes such as cyclin B and polo-like kinase (Plk). Our results support the important role of forkhead in the control of mammalian cell cycle completion, and suggest that efficient execution of the mitotic programme depends on downregulation of PI(3)K/PKB and consequent induction of FKH transcriptional activity. |
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Authors:
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B Alvarez; C Martínez-A; B M Burgering; A C Carrera |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nature Volume: 413 ISSN: 0028-0836 ISO Abbreviation: Nature Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-10-18 Completed Date: 2001-12-05 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 0410462 Medline TA: Nature Country: England |
Other Details:
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Languages: eng Pagination: 744-7 Citation Subset: IM |
Affiliation:
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Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid E-28049, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Cell Cycle Proteins Cyclin B / genetics* DNA-Binding Proteins / metabolism Endoplasmic Reticulum / metabolism Forkhead Transcription Factors G2 Phase Gene Expression Regulation Genes, cdc Humans Mice Mitosis / physiology* Nuclear Proteins / physiology* Phosphatidylinositol 3-Kinases / metabolism* Phosphorylation Protein Kinases / genetics* Protein-Serine-Threonine Kinases* Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-akt Signal Transduction Transcription Factors / metabolism, physiology* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Cyclin B; 0/DNA-Binding Proteins; 0/FOXO1 protein, human; 0/Forkhead Transcription Factors; 0/Nuclear Proteins; 0/Proto-Oncogene Proteins; 0/Transcription Factors; EC 2.7.-/Protein Kinases; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.1/polo-like kinase 1 |
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